物料
polyester film
无菌性
non-sterile
包装
pack of 100 ea
制造商/商品名称
Excel Scientific TS-RT2RR-100
技术
PCR: suitable
qPCR: suitable
长度
130.6 mm
宽度 × 长度
7.78 cm × 13.08 cm
宽度
77.8 mm
颜色
clear
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一般描述
A combination of optically transparent polyester film with a strong, ultra-smooth, non-absorbing, non-fluorescing medical-grade adhesive for superior performance in real-time PCR applications. In place of the customary paper backing, ThermalSeal RT has a plastic liner which is easily removed before use and contributes smoothness and extreme optical clarity to the adhesive. Dimensions are 3.125 x 5.625 in. to seal PCR plates. Length between the perforated ends is 4.8 in.
- Ultra-high optical clarity
- Heat resistant, recommended for temperatures from -40°C to +120°C
- Certified DNase, RNase, and nucleic acid free
ThermalSeal RT2RR™ 50 μm polyester sealing films for real-time PCR are sized to fit within the edges of raised-rim 96 well plates. The same consistent ultra-high optical clarity as Excel′s ThermalSeal RT™ sealing films makes possible more reproducible, reliable, and consistent DNA amplification measurements. An inert, strong, temperature-resistant adhesive assures reliable sealing around each well. Two end tabs assist in positioning the sealing film. Easy removal of the end tabs at perforated boundaries prevents lifting and higher evaporation rates that can occur with sealing films that overlap the plate rim. Recommended for specific DNA sequence detection in clinical diagnostics, forensic science and basic research.
Dimensions 77.8 by 130.8 mm. With end tabs removed, length is 118.1 mm with 45° corners.
Dimensions 77.8 by 130.8 mm. With end tabs removed, length is 118.1 mm with 45° corners.
- Ultra-high optical clarity
- Fit within plate rim to prevent evaporation due to sealing film lifting
- Heat resistant, recommended for temperatures from
- -40 °C to +120 °C
- Certified DNase, RNase, and Nucleic Acid free
法律信息
ThermalSeal is a registered trademark of Excel Scientific, Inc.
ThermalSeal RT is a trademark of Excel Scientific, Inc.
ThermalSeal RT2RR is a trademark of Excel Scientific, Inc.
实验方案
Method for purification, reverse transcription and quantitative PCR for MicroRNAs using Mysticq reagents
Perform reverse transcription (RT) using a reverse transcriptase enzyme and dNTPs. Use total RNA or a gene-specific approach so that only the RNA of interest is converted to cDNA.
Reverse transcription analyzes mRNA gene expression, facing challenges like transcript half-life differences and temporal patterns.
The 3’/5’ integrity assay identifies RNA degradation, crucial for large sample sets or less detectable degradation.
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