Anti-USP30 Antibody, clone 2M2-H1 ZooMAb^® Rabbit Monoclonal

Quality Control Testing

Evaluated by Western Blotting in U-2 OS cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected USP30 in U-2 OS cell lysate.

Tested Applications

Affinity Binding Assay: A representative lot of this antibody bound USP30 peptide with at least one million-fold (1,000,000X) higher affinity than with non-specific control peptide.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected USP30 in HeLa cells.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected USP30 in human kidney tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Clone 2M2-H1 is a ZooMAb^® recombinant rabbit monoclonal antibody that specifically detects USP30. It targets an epitope within 24 amino acids from the C-terminal half, cytoplasmic domain.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Ubiquitin carboxyl-terminal hydrolase 30 (UniProt: Q70CQ3; also known as EC:3.4.19.12, Deubiquitinating enzyme 30, Ubiquitin thioesterase 30, Ubiquitin-specific-processing protease 30, Ub-specific protease 30) is encoded by the USP30 gene (Gene ID: 84749) mapped to human chromosome 12q24.11. The encoded protein belongs to the ubiquitin-specific protease family. It is a deubiquitinating protein localized in the outer membrane of mitochondria. It is mainly involved in the maintenance of mitochondrial morphology through the deubiquitination. It has been reported that USP30 plays an important role in the Parkinson s disease. Overexpressed USP30 generates damaged mitochondria and blocks parkin s ability to mediate mitophagy by removing parkin attached ubiquitin. On the other end, reduced activity of USP30 causes enhanced mitochondrial degradation in neurons. Research data has concluded that inhibited expression of USP30 plays a positive role in the Parkinson s disease by promoting mitochondrial clearance and quality control. This ZooMAb^® recombinant monoclonal antibody, generated by our proprietary technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.

We are committed to bringing you greener alternative products, which adhere to one or more of the 12 Principles of Green Chemistry. This antibody is preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed, and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

KLH-conjugated linear peptide corresponding to 24 amino acids from the C-terminal half, cytoplasmic domain of human USP30.

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany