Anti-G9a Antibody, clone 1N3 ZooMAb^® Rabbit Monoclonal

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Histone-lysine N-methyltransferase EHMT2 (UniProt: Q96KQ7; also known as Euchromatic histone-lysine N-methyltransferase 2, HLA-B-associated transcript 8, Histone H3-K9 methyltransferase 3 (H3-K9-HMTase 3), Lysine N-methyltransferase 1C, Protein G9a, EC 2.1.1.367) is encoded by the EHMT2 (also known as BAT8, C6orf30, G9A, KMT1C, NG36) gene (Gene ID: 10919) in human.
Histone-lysine N-methyltransferase EHMT2 (G9a) is a protein lysine methyltransferase that catalyzes mono- and di-methylation of histone H3 at lysine 9 (H3K9me1 and H3K9me2). This modification is associated with transcriptional repression and epigenetic regulation of gene expression. EHMT2 contains a cysteine-rich region (CRR) with an embedded RING domain, as revealed by X-ray crystallography, which plays a role in its catalytic activity and protein interactions. In addition to histones, EHMT2 methylates non-histone proteins such as p53, influencing cellular processes like tumorigenesis and apoptosis. EHMT2 is essential for embryogenesis, as genetic deletion in mouse models results in embryonic lethality. Its dysregulation is implicated in various cancers, including hepatocellular carcinoma, neuroblastoma, and lung cancer. EHMT2 contributes to tumorigenesis by epigenetically silencing tumor suppressor genes through promoter methylation. In hepatocellular carcinoma, EHMT2 suppresses APC expression, promoting Wnt/β-catenin pathway activation. This ZooMAb^® recombinant monoclonal antibody, generated by our proprietary technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Yuan Guo et al., (2021), Cell & Bioscience, 11, Article 152).

KLH-conjugated linear peptide corresponding to 18 amino acids from the C-terminal half of human G9a.

Quality Control Testing

Evaluated by Immunohistochemistry in human colon tissue sections.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected G9a in human colon tissue sections.

Tested Applications

Affinity Binding Assay: A representative lot of this antibody bound with G9a peptide with at least one hundred thousand-fold (100,000X) higher affinity than with non-specific control peptide.

Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected G9a in MCF7 cells.

Note: Actual optimal working dilutions must be determined by end user as specimens and experimental conditions may vary with the end user.

Clone 1N3 is a ZooMAb^® rabbit recombinant monoclonal antibody that detects G9a. It targets an epitope within 18 amino acids from the C-terminal half.

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.