Anti-Caspase-6 Antibody, clone 3I12 ZooMAb^® Rabbit Monoclonal

Quality Control Testing

Evaluated by Western Blotting in Jurkat cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected p18 subunit and full-length Caspase-6 in Jurkat cell lysate.

Tested Applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected p18 subunit and full-length Caspase-6 in lysates from MOLT-4, NIH/3T3, and ACHN cells.

Enzyme Immunoassay: A representative lot of this antibody detected His-tagged, full-length, recombinant, human Caspase-6 in ELISA application.

Affinity Binding Assay: A representative lot of this antibody bound with Caspase-6 peptide with at least one hundred thousand-fold (100,000X) higher affinity than with non-specific control peptide.

Note: Actual optimal working dilutions must be determined by end user as specimens and experimental conditions may vary with the end user.

Clone 3I12 is a ZooMAb^® rabbit recombinant monoclonal antibody that detects p18 subunit and full-length Caspase-6. It targets an epitope within 18 amino acids from the N-terminal half.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Caspase-6 (UniProt: P55212; also known as EC:3.4.22.59; CASP-6, CSP-6, Apoptotic protease Mch-2) is encoded by the CASP6 (also known as MCH2) gene (Gene ID: 839) in human. Caspase-6 is a cysteine protease classified as an effector caspase, playing key roles in apoptotic and inflammatory programmed cell death pathways, including apoptosis, pyroptosis, necroptosis, and the combined process known as PANoptosis. CASP6 is expressed as a dimeric zymogen composed of a short pro-domain (p11) and a large subunit (p18), with activation requiring cleavage at specific sites. Its activity is tightly regulated by upstream initiator caspases, particularly caspase-8, and by interactions with cellular scaffolding proteins such as RIPK3 and ZBP1, which mediate inflammasome activation and innate immune responses. Caspase-6 is involved in the degradation of key cellular targets, such as lamin A, which contributes to nuclear disassembly during apoptosis. Its roles extend to innate immunity, where it cleaves immunosuppressive molecules like interleukin-10 and IRAK-M, thus promoting immune activation. Caspase-6 is also implicated in neurodegenerative diseases through its cleavage of mutant huntingtin (mHTT) protein in Huntington s disease and amyloid precursor protein (APP) in Alzheimer s disease, leading to toxic protein aggregates that exacerbate disease progression. Caspase-6 is predominantly localized to the cytosol, although its activity is spatially regulated within cellular compartments during apoptosis and inflammatory responses. Emerging evidence highlights Caspase-6′s ability to regulate cancer cell invasion by modulating matrix metalloproteinases (MMP-2 and MMP-9), suggesting a broader involvement in tumor progression and metastasis. This ZooMAb^® recombinant monoclonal antibody, generated by our proprietary technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.

KLH-conjugated linear peptide corresponding to 18 amino acids from the N-terminal half of human Caspase-6.

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany