Anti-TIMP3 Antibody, clone 1H8 ZooMAb^® Rabbit Monoclonal

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Metalloproteinase inhibitor 3 (UniProt: P35625; also known as Protein MIG-5, Tissue inhibitor of metalloproteinases 3, TIMP-3) is encoded by the TIMP3 gene (Gene ID: 7078) in human. The tissue inhibitors of metalloproteinases (TIMPs) represent a family of ubiquitous proteins that are natural inhibitors of the matrix metalloproteinases (MMPs). There are four known members of the TIMP family that are designated as TIMP-1, 2, 3, and 4. TIMPs are released during tissue damage to facilitate tissue remodeling through degradation and reorganization of the extracellular matrix. During this process MMPs are involved in inflammatory response through proteolytic maturation of cytokines. These activities are regulated through a 1:1 stoichiometric interaction of MMPs with TIMPs and these interactions are tightly controlled. TIMP3 is synthesized with a signal peptide (aa 1-23), which is subsequently cleaved off to produce the mature form. It mediates a variety of processes including matrix regulation and turnover, inflammation, and angiogenesis, through reversible inhibition of MMPs. It is also reported to modulate the processing of amyloid precursor protein and apolipoprotein E receptor ApoER2 by inhibiting two -secretases (ADAM10 and ADAM17). TIMP3 also functions as a tumor suppressor and a potent inhibitor of angiogenesis. It exerts its anti-angiogenic effect by directly interacting with VEGF receptor-2/KDR, preventing its binding to the VEGFA ligand. This ZooMAb^® recombinant monoclonal antibody, generated by our proprietary technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Hoe, H-S., et al. (2007). J. Neurosci. 27(40); 10895-10905; Qi, JH., et al. (2003). Nat. Med. 9(4); 407-415).

KLH-conjugated linear peptide corresponding to 20 amino acids from the C-terminal region of human TIMP3.

Quality Control Testing
Evaluated by Western Blotting in mouse kidney tissue lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected TIMP3 in mouse kidney tissue lysate.
Tested Applications
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected TIMP3 in lysate from SK-MEL-28 cells.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected TIMP3 in human placenta tissue sections.

Affinity Binding Assay: A representative lot of this antibody bound TIMP3 peptide with at least ten million-fold (10,000,000X) higher affinity than with non-specific control peptide.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Clone 1H8 is a ZooMAb^® rabbit recombinant monoclonal antibody that specifically detects TIMP3. It targets an epitope within 20 amino acids from the C-terminal region.

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.