Anti-HBA1/HBA2 Antibody, clone 3I10 ZooMAb^® Rabbit Monoclonal

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.
Hemoglobin subunit alpha (UniProt: P69905; also known as Alpha-globin, Hemoglobin alpha chain) is encoded by the HBA1 (also known as HBA2) gene (Gene ID: 3039, 3040) in human. Hemoglobin subunit alpha is a critical component of the hemoglobin molecule, responsible for oxygen transport in red blood cells. It is a globular protein with eight alpha-helical segments that form a compact, stable structure. Each hemoglobin molecule is a tetramer consisting of two alpha (HBA1) and two beta subunits, coordinating iron-containing heme groups that reversibly bind oxygen. Hemoglobin subunit alpha is involved in both fetal hemoglobin (HbF, alpha-gamma composition) and adult hemoglobin (HbA, alpha-beta composition), ensuring oxygen delivery throughout different developmental stages. HBA1 is localized in erythrocytes and functions in essential biochemical pathways such as oxygen transport, carbon dioxide release, and buffering pH in blood. Mutations or deletions in the HBA1 gene are associated with disorders like alpha-thalassemia, where reduced or absent alpha-globin chain production leads to imbalanced globin chain synthesis. This condition manifests in varying severity, from asymptomatic carriers to life-threatening conditions like Hb Bart syndrome, characterized by severe anemia and hypoxia. Other pathologies include methemoglobinemia (excessive oxidation of heme iron) and erythrocytosis, which alters oxygen affinity. The interaction of HBA1 with beta-globin (HBB) subunits forms the basis of hemoglobin′s cooperative oxygen-binding mechanism, mediated by allosteric transitions between relaxed (R) and tense (T) states. Dysregulation of these mechanisms, as seen in hemoglobinopathies, contributes to tissue hypoxia, oxidative stress, and systemic complications. Recent studies also implicate alpha-globin in non-erythroid tissues, suggesting roles in oxidative metabolism and cellular protection. This ZooMAb^® recombinant monoclonal antibody, generated by our proprietary technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Ahmed M.H., et al., (2020), Subcell Biochem, 94, 345-382)

KLH-conjugated linear peptide corresponding to 16 amino acids from the internal region of human HBA1/HBA2.

Quality Control Testing
Evaluated by Western Blotting in human heart tissue lysate. 

Western Blotting Analysis: A 1:10,000 dilution of this antibody detected HBA1/HBA2 in human heart tissue lysate.
Tested Applications
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected HBA1/HBA2 in human placenta tissue lysate.
Affinity Binding Assay: A representative lot of this antibody bound with HBA1/HBA2 peptide with at least one thousand-fold (1,000X) higher affinity than with non-specific control peptide.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected HBA1/HBA2 in human placenta tissue sections.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Clone 3I10 is a ZooMAb^® rabbit recombinant monoclonal antibody that detects HBA1/HBA2. It targets an epitope within 16 amino acids from the internal region.

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.