跳转至内容
Merck
CN
登录查看公司和协议定价

About This Item

UNSPSC代码:
41115712
NACRES:
NB.21
技术服务
需要帮助?我们经验丰富的科学家团队随时乐意为您服务。
让我们为您提供帮助

Product Name

HybridSPE ®-磷脂SPE小柱, Cartridge, bed wt. 30 mg, volume 1 mL, pk of 200, polypropylene material (hardware), PE frit (20 μm)

物料

PE frit (20 μm)
polypropylene hardware

质量水平

组成

bed wt., 30 mg

包装

pk of 200

技术

solid phase extraction (SPE): suitable

体积

1 mL

基质活性基团

zirconia-based phase

正在寻找类似产品? 访问 产品对比指南

一般描述

HybridSPE-Phospholipid磷脂去除技术是一种简单且通用的样本制备平台,设计用于在 LC-MS 或 LC-MS/MS 分析之前,从生物血浆和血清中总体去除内源性蛋白和磷脂干扰。首先通过加入和混合酸化乙腈使生物血浆或血清发生蛋白沉淀。然后通过离心除去沉淀蛋白,并将所得上清液上样至 HybridSPE-Phospholipid 96 孔板或固相萃取柱上,其作为化学过滤器,专门靶向去除内源性样品磷脂。截留磷脂的原理是HybridSPE-Phospholipid固定相键合的专有氧化锆离子和所有磷脂都具有的磷酸根发生高度选择性的路易斯酸碱相互作用。得到的洗脱液可立即用于 LC-MS 或 LC-MS-MS 分析。

HybridSPE-Phospholipid 96 孔板和Ultra固相萃取柱有“孔内”/“柱内”沉淀法可供选择。即先将生物血浆/血清加入孔内或柱内,接着加入酸化乙腈(沉淀剂)。短暂混合/涡旋后,抽真空。96孔板和 Ultra固相萃取柱含有一系列低孔隙率疏水滤膜/筛板,这些填充柱床的滤膜/筛板组件作为深层过滤器,有助于在提取过程中同时去除磷脂和沉淀蛋白。标准 HybridSPE-Phospholipid磷脂固相萃取小柱需要采用“离柱”沉淀法。

应用


  • Less is more: a methodological assessment of extraction techniques for per- and polyfluoroalkyl substances (PFAS) analysis in mammalian tissues.: This study evaluates various extraction techniques, including the use of HybridSPE-Phospholipid, for analyzing PFAS in mammalian tissues. The research highlights the importance of effective phospholipid removal to ensure accurate PFAS quantification (Mertens et al., 2023).

  • Rapid analysis of 65 pharmaceuticals and 7 personal care products in plasma and whole-body tissue samples of fish using acidic extraction, zirconia-coated silica cleanup, and liquid chromatography-tandem mass spectrometry.: The study includes HybridSPE-Phospholipid as part of the cleanup process, demonstrating its efficiency in removing phospholipids to improve the detection of pharmaceuticals and personal care products in biological samples (Tanoue et al., 2020).

  • Multi LC-MS/MS and LC-HRMS Methods for Determination of 24 Mycotoxins including Major Phase I and II Biomarker Metabolites in Biological Matrices from Pigs and Broiler Chickens.: This research utilizes HybridSPE-Phospholipid for the cleanup of biological matrices, enhancing the accuracy of mycotoxin detection by effectively removing interfering phospholipids (Lauwers et al., 2019).

  • Effective phospholipid removal from plasma samples by solid phase extraction with the use of copper (II) modified silica gel cartridges.: The study compares various phospholipid removal techniques, highlighting the performance of HybridSPE-Phospholipid in achieving high phospholipid removal efficiency from plasma samples (Flieger et al., 2017).

  • Liquid chromatography mass spectrometry determination of perfluoroalkyl acids in environmental solid extracts after phospholipid removal and on-line turbulent flow chromatography purification.: The research demonstrates the application of HybridSPE-Phospholipid in the removal of phospholipids from environmental solid extracts, facilitating the accurate measurement of perfluoroalkyl acids (Mazzoni et al., 2016).

特点和优势

  • 融合了蛋白质沉淀的简单性和固相萃取(SPE)定向除去磷脂的选择性
  • 通过完全除去磷脂和沉淀蛋白,减少离子抑制
  • 2-3 步通用流程
  • 方法开发最少,甚至不需要方法开发
  • 有 96 孔和 1 mL 萃取柱规格可供选择

法律信息

HybridSPE is a registered trademark of Merck KGaA, Darmstadt, Germany

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable


历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

没有发现合适的版本?

如果您需要特殊版本,可通过批号或批次号查找具体证书。

已有该产品?

在文件库中查找您最近购买产品的文档。

访问文档库

我们的科学家团队拥有各种研究领域经验,包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

联系客户支持