产品名称
apHera ™ NH 2 HPLC 色谱柱, 5 μm particle size, L × I.D. 25 cm × 4.6 mm
material
stainless steel column
agency
suitable for USP L82
product line
apHera™
packaging
pkg of 1 ea
manufacturer/tradename
apHera™
parameter
147 bar pressure (2133 psi)
4-50 °C temperature
technique(s)
HPLC: suitable
LC/MS: suitable
L × I.D.
25 cm × 4.6 mm
matrix
polymeric
matrix active group
amino phase
particle size
5 μm
pore size
300 Å
operating pH
1-14
application(s)
food and beverages
separation technique
hydrophilic interaction (HILIC)
normal phase
Quality Level
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General description
apHera™氨基柱是基于专为分离单糖和寡糖而优化的共价键合多胺。单糖、二糖、三糖的洗脱顺序显示出锁着乙腈浓度增加的洗脱体积增加，以及酸性和碱性洗脱液的完全稳定性。小而坚固的PVA共聚物珠可提供机械和化学强度以及较高的柱效率。基于二氧化硅的常规折射柱并没有显示出长的柱寿命，可能是由于碱性氨基对二氧化硅颗粒的水解。由于Supelco使用了一种可兼容强碱性的聚合物，因而消除了这些问题。 稳定的保留时间和长的色谱柱寿命也是色谱柱的特征。
Amino apHera™色谱柱特性
Amino apHera™色谱柱特性
- 高效的碳水化合物分析
- 适用于碱性条件/两性检测
- 单至寡糖的高选择性
- pH范围2-13
- 300埃孔径
- 保留稳定、可预测
- 色谱柱寿命长
Legal Information
apHera is a trademark of Sigma-Aldrich Co. LLC
Khin Than Myint et al.
Analytical chemistry, 81(18), 7766-7772 (2009-08-26)
We have developed a practical method for the comprehensive analysis of polar anionic metabolites in biological samples with the use of a nano-LC/MS system. A polyamine-bonded polymer-based apHera NH2 column, which is compatible with ammonium carbonate buffer, effectively retained anionic
Marine de Person et al.
Journal of chromatography. A, 1081(2), 174-181 (2005-07-26)
A new procedure based on hydrophilic interaction chromatography coupled to tandem mass spectrometry (ionisation process by pneumatically assisted electrospray in negative ion mode), is developed and validated for the simultaneous determination of underivatised taurine and methionine in beverages rich in
相关内容
apHera Amino HPLC Columns
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