产品名称
Ascentis® RP-Amide HPLC 色谱柱, 5 μm particle size, L × I.D. 25 cm × 4.6 mm
物料
stainless steel column
质量水平
Agency
suitable for USP L60
产品线
Ascentis®
特点
endcapped
制造商/商品名称
Ascentis®
包装
1 ea of
标记范围
19.5% Carbon loading
参数
≤70 °C temp. range
400 bar pressure (5801 psi)
技术
HPLC: suitable
LC/MS: suitable
长度 × 内径
25 cm × 4.6 mm
表面积
450 m2/g
表面覆盖度
2.7 μmol/m2
杂质
<5 ppm metals
基质
fully porous particle
silica gel high purity, spherical
基质活性基团
amide, alkyl phase
粒径
5 μm
孔径
100 Å
工作pH范围
2-8
应用
environmental
food and beverages
分离技术
reversed phase
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一般描述
Ascentis RP-Amide 反相酰胺柱是新一代超低流失、嵌入极性基团 (EPG) 的键合相，该键合相可提供与常规C18不同的选择性，并提高极性化合物 HPLC 和 LC-MS 分析的分离度。Ascentis RP-Amide 反相酰胺柱是嵌入极性基团 HPLC 键合相的首选。
应用
- A validated UHPLC method for the determination of caffeoylquinic and di-caffeoylquinic acids in green coffee extracts using an RP-Amide fused-core column.: 本研究介绍了一种经过验证的超高效液相色谱 (UHPLC) 方法，该方法采用 RP-Amide 熔融核色谱柱定量测定生咖啡提取物中的咖啡酰奎宁和二咖啡酰奎宁酸。该方法表现出极好的精度、准确度和灵敏度，因而成为分析复杂基质中这些生物活性化合物的宝贵工具。该研究重点介绍了 Ascentis® RP-Amide HPLC 色谱柱在提高分析化学应用中色谱分离度和效率方面的实用性 (Fibigr et al., 2018)。
特点和优势
- 对极性化合物具有良好的保留性能和峰形
- 100% 水兼容
- 超低流失，与 LC-MS 兼容
- 独特的选择性
法律信息
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
Can I use Ascentis Express on a UHPLC system?
Yes. Ascentis Express columns are packed in a way making them suitable for these ultra high pressure instruments. In fact, Ascentis Express outperforms sub-2 μm micron columns on many applications since Ascentis Express provides the benefits of sub-2 μm particles but at much lower back pressure. These benefits include the capability of providing fast HPLC and higher resolution chromatography. The Fused-Core particle consists of a 1.7 μm solid core and a 0.5 μm porous shell. A major benefit of the Fused-Core particle is the small diffusion path (0.5 μm) compared to conventional fully porous particles. The shorter diffusion path reduces axial dispersion of solutes and minimizes peak broadening.
What solvent should Product No. 565325-U Ascentis RP-Amide HPLC Column be stored in?
Product No. 565325-U should be stored in 100% acetonitrile after any buffers have been flushed out.
What is the Product No of the guard column for Product No. 565325-U Ascentis RP-Amide HPLC column?
The guard column kit for Product No. 565325-U is 565371-U. This kit includes one cartridge, stand-alone holder, a piece of tubing, 2 nuts and 2 ferrules. A set of two replacement cartridges is Product No. 565370-U.
How do I find price and availability?
There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 or view local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
My question is not addressed here, how can I contact Technical Service for assistance?
Ask a Scientist here.
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
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