565325-U
Ascentis® RP-Amide (5 µm) HPLC Columns
L × I.D. 25 cm × 4.6 mm, HPLC Column
别名:
RP-Amide反相色谱柱
选择尺寸
关于此项目
silica gel high purity, spherical
产品名称
Ascentis® RP-Amide HPLC 色谱柱, 5 μm particle size, L × I.D. 25 cm × 4.6 mm
material
stainless steel column
agency
suitable for USP L60
product line
Ascentis®
feature
endcapped
manufacturer/tradename
Ascentis®
packaging
1 ea of
extent of labeling
19.5% Carbon loading
parameter
≤70 °C temp. range
400 bar pressure (5801 psi)
technique(s)
HPLC: suitable
LC/MS: suitable
L × I.D.
25 cm × 4.6 mm
surface area
450 m2/g
surface coverage
2.7 μmol/m2
impurities
<5 ppm metals
matrix
fully porous particle
silica gel high purity, spherical
matrix active group
amide, alkyl phase
particle size
5 μm
pore size
100 Å
operating pH range
2-8
application(s)
environmental
food and beverages
separation technique
reversed phase
Quality Level
正在寻找类似产品? 访问 产品对比指南
Legal Information
Application
- A validated UHPLC method for the determination of caffeoylquinic and di-caffeoylquinic acids in green coffee extracts using an RP-Amide fused-core column.: 本研究介绍了一种经过验证的超高效液相色谱 (UHPLC) 方法，该方法采用 RP-Amide 熔融核色谱柱定量测定生咖啡提取物中的咖啡酰奎宁和二咖啡酰奎宁酸。该方法表现出极好的精度、准确度和灵敏度，因而成为分析复杂基质中这些生物活性化合物的宝贵工具。该研究重点介绍了 Ascentis® RP-Amide HPLC 色谱柱在提高分析化学应用中色谱分离度和效率方面的实用性 (Fibigr et al., 2018)。
Features and Benefits
- 对极性化合物具有良好的保留性能和峰形
- 100% 水兼容
- 超低流失，与 LC-MS 兼容
- 独特的选择性
General description
Ascentis RP-Amide 反相酰胺柱是新一代超低流失、嵌入极性基团 (EPG) 的键合相，该键合相可提供与常规C18不同的选择性，并提高极性化合物 HPLC 和 LC-MS 分析的分离度。Ascentis RP-Amide 反相酰胺柱是嵌入极性基团 HPLC 键合相的首选。
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
Can I use Ascentis Express on a UHPLC system?
Yes. Ascentis Express columns are packed in a way making them suitable for these ultra high pressure instruments. In fact, Ascentis Express outperforms sub-2 μm micron columns on many applications since Ascentis Express provides the benefits of sub-2 μm particles but at much lower back pressure. These benefits include the capability of providing fast HPLC and higher resolution chromatography. The Fused-Core particle consists of a 1.7 μm solid core and a 0.5 μm porous shell. A major benefit of the Fused-Core particle is the small diffusion path (0.5 μm) compared to conventional fully porous particles. The shorter diffusion path reduces axial dispersion of solutes and minimizes peak broadening.
What solvent should Product No. 565325-U Ascentis RP-Amide HPLC Column be stored in?
Product No. 565325-U should be stored in 100% acetonitrile after any buffers have been flushed out.
What is the Product No of the guard column for Product No. 565325-U Ascentis RP-Amide HPLC column?
The guard column kit for Product No. 565325-U is 565371-U. This kit includes one cartridge, stand-alone holder, a piece of tubing, 2 nuts and 2 ferrules. A set of two replacement cartridges is Product No. 565370-U.
How do I find price and availability?
There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 or view local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
My question is not addressed here, how can I contact Technical Service for assistance?
Ask a Scientist here.
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系客户支持