567507-U
Discovery® HS F5 (3 µm) HPLC Columns
L × I.D. 15 cm × 4.6 mm, HPLC Column
别名:
Discovery五氟苯基PFP HPLC色谱柱
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关于此项目
UNSPSC Code:
41115700
eCl@ss:
32110501
NACRES:
SB.52
L × i.d.:
15 cm × 4.6 mm
Particle size:
3 μm
Matrix active group:
PFP (pentafluorophenyl) phase
Pore size:
120 Å
Matrix:
silica gel, high purity, spherical particle platform
fully porous particle
fully porous particle
产品名称
Discovery ® HS F5 HPLC 色谱柱, 3 μm particle size, L × I.D. 15 cm × 4.6 mm
material
stainless steel column
agency
suitable for USP L43
product line
Discovery®
feature
endcapped
manufacturer/tradename
Discovery®
packaging
1 ea of
extent of labeling
12% Carbon loading
parameter
≤70 °C temp. range
400 bar pressure (5801 psi)
technique(s)
HPLC: suitable
LC/MS: suitable
L × I.D.
15 cm × 4.6 mm
surface area
300 m2/g
surface coverage
4 μmol/m2
impurities
<10 ppm metals
matrix
silica gel, high purity, spherical particle platform
fully porous particle
matrix active group
PFP (pentafluorophenyl) phase
particle size
3 μm
pore size
120 Å
operating pH range
2-8
application(s)
food and beverages
separation technique
reversed phase
Quality Level
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Application
- Nature of the main contaminant in the drug primaquine diphosphate: SFC and SFC-MS methods of analysis.：采用Discovery® HS F5 HPLC色谱柱鉴定和定量二磷酸伯氨喹中的主要污染物，对于确保这种药物的品质和安全性有重要意义(Brondz et al., 2007)。
- HPLC assisted chemobiological standardization of alpha-glucosidase-I enzyme inhibitory constituents from Piper longum Linn-An Indian medicinal plant.：使用Discovery® HS F5 HPLC色谱柱进行印度药用植物的生物相关化学分析，助力草药标准化(Pullela et al., 2006)。
- Disposition study of a new potential antineoplastic agent dimefluron in rats using high-performance liquid chromatography with ultraviolet and mass spectrometric detection.：利用Discovery® HS F5 HPLC色谱柱研究新型抗癌药物的药动学和代谢，助力开发有效的癌症药物(Císar et al., 2005)。
- A simplified analytical method for a phenotyping cocktail of major CYP450 biotransformation routes.：采用Discovery® HS F5 HPLC色谱柱简化分型混合物(phenotyping cocktail)分析，改进药物代谢评估和个性化医疗方法(Jerdi et al., 2004)。
Features and Benefits
- 独特的选择性
- 保留能力与 C18 柱类似（有时需要洗脱能力更强的流动相）
- 峰形极佳
- 稳定性好、低流失，适用于 LC-MS 分析
- 粒径范围从 3μm 到 10μm 可供选择
General description
将 C18 柱方法转换至 Discovery® HS F5 柱的指导原则：
通常，碱性化合物在 HS F5 色谱柱上的保留时间比在 C18 柱上更长。在 C18 柱上流动相中的有机相含量提高 5% 至 10% 通常可获得与 HS F5 柱类似的保留值。使用其他化合物所得的结果差异则很大。然而 log Po/w 值小于 2.5 的化合物在 HS F5 柱上的保留时间通常要长于 C18 柱。保留时间的差异程度与化合物的 logP o/w 值密切相关。
通常，碱性化合物在 HS F5 色谱柱上的保留时间比在 C18 柱上更长。在 C18 柱上流动相中的有机相含量提高 5% 至 10% 通常可获得与 HS F5 柱类似的保留值。使用其他化合物所得的结果差异则很大。然而 log Po/w 值小于 2.5 的化合物在 HS F5 柱上的保留时间通常要长于 C18 柱。保留时间的差异程度与化合物的 logP o/w 值密切相关。
Discovery® HS F5 键合相可提供与 C18 色谱柱截然不同的反相分离。由于 F5 色谱柱上各种化合物通常会在与 C18 相同的时间范围内洗脱出来，使得大部分的 C18 方法可轻松转换到 F5 上。
Legal Information
Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany
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