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Merck
CN

581324-U

Ascentis® C18 (5 µm) HPLC Columns

L × I.D. 15 cm × 4.6 mm, HPLC Column

别名:

Ascentis RP18 HPLC色谱柱

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UNSPSC Code:
41115700
eCl@ss:
32110501
NACRES:
SB.52

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产品名称

Ascentis ® C18 HPLC 色谱柱, 5 μm particle size, L × I.D. 15 cm × 4.6 mm

material

stainless steel column

agency

suitable for USP L1 (Similar to Phenomenex Luna C18)

product line

Ascentis®

feature

endcapped

manufacturer/tradename

Ascentis®

packaging

1 ea of

extent of labeling

25% Carbon loading

parameter

≤70 °C temp. range
400 bar pressure (5801 psi)

technique(s)

HPLC: suitable
LC/MS: suitable

L × I.D.

15 cm × 4.6 mm

surface area

450 m2/g

surface coverage

3.7 μmol/m2

impurities

<5 ppm metals

matrix

fully porous particle
silica gel high purity, spherical

matrix active group

C18 (octadecyl) phase

particle size

5 μm

pore size

100 Å

operating pH range

2-8

application(s)

food and beverages

separation technique

reversed phase

Quality Level

100

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相关类别

Application


  • Preparation of polar embedded C18 stationary phase for efficient separation of peptides and proteins in high performance liquid chromatography.: 本研究详细介绍了极性嵌入式 C18 固定相的开发，证明了其在分离肽和蛋白质方面的有效性。Ascentis® C18 HPLC色谱柱凭借其高分离效率而得到广泛使用——这对于蛋白质组学和生物制药应用至关重要(Ali et al., 2022)。

  • A dilute-and-shoot liquid chromatography-tandem mass spectrometry method for urinary 18-hydroxycortisol quantification and its application in establishing reference intervals.: 研究人员使用 Ascentis® C18 HPLC 色谱柱开发了一种灵敏的尿液 18-羟基皮质醇定量测定方法。该方法通过提供准确的参考区间，帮助开展临床诊断和内分泌学研究 (Zhao et al., 2022)。

  • A Comparative Study on Analysis of Ginsenosides in American Ginseng Root Residue by HPLC-DAD-ESI-MS and UPLC-HRMS-MS/MS.: 本比较分析利用Ascentis® C18 HPLC色谱柱分离和鉴定西洋参中的人参皂苷。 本研究重点介绍了该色谱柱在制药行业天然产物研究和质量控制中的应用(Hsu et al., 2022)。

  • Validation of a Rapid and Easy-to-Apply Method to Simultaneously Quantify Co-Loaded Dexamethasone and Melatonin PLGA Microspheres by HPLC-UV: Encapsulation Efficiency and In Vitro Release.: 本研究验证了一种使用Ascentis® C18 色谱柱同时HPLC定量 PLGA 微球中地塞米松和褪黑激素的 方法，为药物输送研究提供了强大的工具(Brugnera et al., 2022)。

  • Simultaneous determination of ivermectin, clorsulon and their related substances in an injectable finished product by a stability-indicating RP-HPLC method.: 采用Ascentis® C18 HPLC色谱柱开发一种稳定性指示方法，用于分析药物制剂中的伊维菌素和氯舒隆，凸显其在质量控制和法规遵从性方面的作用(Padivitage et al., 2022)。

Features and Benefits

  • 出色的保留性能
  • 优良的峰形对称性
  • 重现性好
  • 与 LC-MS 完全兼容

General description

Ascentis 系列色谱柱是 Supelco 科学家推出的第四代 HPLC 色谱柱技术。Ascentis 系列色谱柱是以高纯硅胶为基质，孔径 100Å，硅胶粒径为 3、5 和 10 微米。该色谱柱用于小分子的应用，柱内径可从微径柱（1.0mm 内径）扩展到制备尺寸（50mm 内径）。该系列包括 C18、C8、苯基、Si 和内嵌极性基团相（反相酰胺）。

Ascentis C18 是特别稳定可靠的首选 HPLC 色谱柱，即使对于难以分离的化合物也能提供对称的峰形和出色的保留性能。

Other Notes

探索最适于 HPLC 或 LC-MS 分析的 LiChropur 试剂

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

存储类别

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
297520-06
297520-05
297520-04
297520-03
297520-02

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the storage solvent for Product 581324-U, Ascentis® C18 HPLC Column - 5 ?m particle size, L × I.D. 15 cm × 4.6 mm?

    The storage solvent is a ratio of 40:60 water:acetonitrile for this column.

  5. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  6. What flow rate should I use with Ascentis® Express HPLC Columns?

    Based on the minimum in the van Deemter curves, higher flows than 5um particle columns are required in order to maximize Ascentis Express column efficiency. The suggested starting point for flow rate for Ascentis Express columns: 1.6 mL/min for 4.6 mm ID;  0.8 mL/min for 3.0 mm ID; and 0.4mL/min for 2.1 mm ID.

  7. Can Ascentis® Express HPLC Columns be used for LC-MS?

    Express Fused-Core™ particles were designed with LC-MS in mind. Even extremely short column lengths exhibit sufficient plate counts to show high resolving power. The flat van Deemter plots permit resolution to be maintained at very high flow rates to maximize sample throughput. All Ascentis stationary phases have been evaluated for MS compatibility during their development, and the Express phases are no exception. You can expect extremely low column bleed and background while maintaining longest possible column lifetime. A bonus of Ascentis Express columns for high throughput UHPLC and LC-MS is that they are extremely rugged and highly resistant to plugging, a very common failure mode for competitor columns.

  8. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Dipanjan Goswami et al.
Biomedical chromatography : BMC, 23(11), 1227-1241 (2009-07-14)
A LC-MS/MS method for plasma topiramate analysis is delineated involving least number of healthy volunteers. Topiramate and amlodipine internal standard (IS) were extracted by simple centrifuge-coupled solid-phase extraction and reverse-phase chromatographic separation was performed on an Ascentis C(18) column. Turbo-spray
Federica Pellati et al.
Journal of pharmaceutical and biomedical analysis, 55(5), 934-948 (2011-04-19)
In this study, the composition of polyphenols (phenolic acids and flavonoids) in propolis extracts was investigated by HPLC-DAD and HPLC-ESI-MS/MS by comparing the performance of ion trap and triple quadrupole mass analyzers. The analyses were carried out on an Ascentis
Federica Vacondio et al.
Journal of pharmaceutical and biomedical analysis, 46(1), 200-205 (2007-10-26)
A rapid, simple and sensitive liquid chromatography-mass spectrometry (LC-MS) method was developed and validated for the determination of the imidazole H(3) antagonist ROS203 in rat plasma, using the superior homologue ROS287 as internal standard. Analyses were performed on an Agilent
Federica Pellati et al.
Journal of chromatography. A, 1242, 43-58 (2012-05-09)
In this study, a detailed phytochemical characterization of Echinacea pallida (Nutt.) Nutt. root extracts and dietary supplements was carried out for the first time by developing advanced chromatographic techniques, based on HPLC with diode array (DAD) and electrospray ionization-mass spectrometry
Meiyun Shi et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 951-952, 129-134 (2014-02-22)
Aralia mandshrica is a well-known traditional Chinese medicine from Northeast China commonly used to treat digestive, circulatory and immune system disorders. Calenduloside E is one of its bioactive components currently under evaluation as a pure drug. In this study, a
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