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关于此项目
产品名称
Ascentis ® C18 HPLC 色谱柱, 5 μm particle size, L × I.D. 25 cm × 4.6 mm
Quality Segment
material
stainless steel column
agency
suitable for USP L1 (Similar to Phenomenex Luna C18)
product line
Ascentis®
feature
endcapped
analyte functional class(es)
antibiotics, explosives, fat soluble vitamins, lipids, nutraceuticals and antioxidants, oils, pigments, sweeteners, water soluble vitamins
analyte chemical class(es)
PAHs, PCBs (aroclor identification), PFAS, aflatoxins, alcohols / fermentation, alcohols and aldehyde, aliphatic amines, alkaloids, amino acids, aromatic amines, cannabinoids as tms derivatives, carotenoids, catecholamines, coloring compounds, essential oils, esters, fatty acids, flavonoids, glycans, ketones, metabolized steroids, nitrosamines, nucleic acids, nucleosides, oligonucleotides, organic phosphates, peptides, pesticides, phenols, phospholipids, phthalate esters, preservatives, proteins, steroids (cholesteryl esters), sulfonamides
manufacturer/tradename
Ascentis®
packaging
1 ea of
extent of labeling
25% Carbon loading
parameter
≤70 °C temp. range, 400 bar pressure (5801 psi)
technique(s)
HPLC: suitable, LC/MS: suitable
L × I.D.
25 cm × 4.6 mm
surface area
450 m2/g
surface coverage
3.7 μmol/m2
impurities
<5 ppm metals
matrix
fully porous particle, silica gel high purity, spherical
matrix active group
C18 (RP18), C18 (octadecyl) phase
particle size
5 μm
pore size
100 Å
operating pH range
2-8
application(s)
food and beverages
separation technique
reversed phase
General description
Ascentis C18 是特别稳定可靠的首选 HPLC 色谱柱,即使对于难以分离的化合物也能提供对称的峰形和出色的保留性能。
Application
- Identification of recombinant human insulin and biosynthetic insulin analogues by multiplexed targeted unlabeled mass spectrometry of proteotypic tryptic peptides.: 本研究利用 Ascentis® C18 HPLC 色谱柱分离蛋白型胰蛋白酶肽,以鉴定胰岛素类似物,重点介绍了其在靶向质谱法进行蛋白质组学分析中的应用(Qasem RJ et al., 2019).。
- Development and validation of a novel stability-indicating HPLC method for the simultaneous assay of betamethasone-17-valerate, fusidic acid, potassium sorbate, methylparaben and propylparaben in a topical cream preparation.: 本研究开发并验证了一种使用 Ascentis® C18 HPLC 色谱柱同时测定多种化合物的 HPLC 方法,证明了其在药物制剂稳定性测试中的稳健性(Byrne J et al., 2014)。
- HPLC-DAD and HPLC-ESI-MS/MS methods for metabolite profiling of propolis extracts.: 本研究采用 Ascentis® C18 HPLC 色谱柱,使用 HPLC-DAD 和 HPLC-ESI-MS/MS 对蜂胶提取物代谢物进行分析,证明了其在复杂天然产物分析中的有效性 (Pellati F et al., 2011)。
- Practical comparison of 2.7 microm fused-core silica particles and porous sub-2 microm particles for fast separations in pharmaceutical process development.: 本文比较了不同的 HPLC 色谱柱技术,包括 Ascentis® C18,重点介绍了其在制药工艺开发中至关重要的快速分离方面的性能 (Abrahim A et al., 2010)。
Features and Benefits
- 出色的保留性能
- 优良的峰形对称性
- 重现性好
- 与 LC-MS 完全兼容
Legal Information
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存储类别
11 - Combustible Solids
WGK
nwg
闪点 (F)
Not applicable
闪点 (°C)
Not applicable