Product Name
Ascentis® C8 HPLC 色谱柱, 3 μm particle size, L × I.D. 5 cm × 2.1 mm
物料
stainless steel column
Agency
suitable for USP L7
产品线
Ascentis®
特点
endcapped
制造商/商品名称
Ascentis®
包装
1 ea of
标记范围
15% Carbon loading
参数
≤70 °C temp. range
400 bar pressure (5801 psi)
技术
HPLC: suitable
LC/MS: suitable
长度 × 内径
5 cm × 2.1 mm
表面积
450 m2/g
表面覆盖度
4.0 μmol/m2
杂质
<5 ppm metals
基质
fully porous particle
silica gel high purity, spherical
基质活性基团
C8 (octyl) phase
粒径
3 μm
孔径
100 Å
工作pH范围
2-8
应用
food and beverages
分离技术
reversed phase
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一般描述
Ascentis 系列色谱柱是 Supelco 科学家推出的第四代 HPLC 色谱柱技术。Ascentis 系列色谱柱是以高纯硅胶为基质，孔径 100Å，硅胶粒径为 3、5 和 10 微米。该色谱柱用于小分子的应用，柱内径可从微径柱（1.0mm 内径）扩展到制备尺寸（50mm 内径）。该系列包括 C18、C8、苯基、Si 和内嵌极性基团相（反相酰胺）。
Ascentis C8 色谱柱重现性好，对极性化合物具有优良的选择性。该键合相在高含水量的流动相和高有机相含量的流动相中均有优良的性能。
Ascentis C8 色谱柱重现性好，对极性化合物具有优良的选择性。该键合相在高含水量的流动相和高有机相含量的流动相中均有优良的性能。
应用
- Analysis of 27 beta-Blockers and Metabolites in Milk Powder by High Performance Liquid Chromatography Coupled to Quadrupole Orbitrap High-Resolution Mass Spectrometry.: This study utilizes the Ascentis® C8 HPLC Column to analyze beta-blockers and their metabolites in milk powder, highlighting the column′s capacity for sensitive and precise quantification in complex matrices (Cheng et al., 2019).
- Recent trends in ultra-fast HPLC: new generation superficially porous silica columns.: A review of advancements in HPLC technology, including the use of Ascentis® C8 columns, which enhance the speed and efficiency of chemical separations (Ali et al., 2012).
- Superficially porous particles columns for super fast HPLC separations.: Discusses the benefits of using Ascentis® C8 HPLC Columns for achieving faster and more efficient separations in HPLC, underlining their utility in high-throughput settings (Ali et al., 2012).
- Gradient RP-HPLC method for the determination of potential impurities in atazanavir sulfate.: Demonstrates the effectiveness of the Ascentis® C8 HPLC Column in a robust method for identifying impurities in pharmaceutical compounds, ensuring product safety and compliance (Chitturi et al., 2011).
- Development and validation of an HPLC-MS/MS method to determine clopidogrel in human plasma. Use of incurred samples to test back-conversion.: Illustrates the application of Ascentis® C8 HPLC Column in a validated method for the analysis of clopidogrel, improving therapeutic drug monitoring and patient care (Silvestro et al., 2010).
特点和优势
- 对疏水性分子的保留性极好
- 峰形极佳
- LC-MS 分离流失低
法律信息
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
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