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Merck
CN

58354

SUPELCOSIL™ LC-8-DB (5 µm) HPLC Columns

L × I.D. 25 cm × 4.6 mm, HPLC Column

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UNSPSC Code:
41115700
eCl@ss:
32110501
NACRES:
NB.21
L × i.d.:
25 cm × 4.6 mm
Particle size:
5 μm
Matrix active group:
C8 (octyl) phase
Pore size:
120 Å
Matrix:
silica gel, spherical particle platform

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产品名称

SUPELCOSIL™ LC-8-DB HPLC 色谱柱, 5 μm particle size, L × I.D. 25 cm × 4.6 mm

agency

suitable for USP L7

feature

endcapped

manufacturer/tradename

SUPELCOSIL™

extent of labeling

6.0% carbon loading

parameter

0-70 °C temperature
400 bar pressure (5801 psi)

technique(s)

HPLC: suitable

L × I.D.

25 cm × 4.6 mm

surface area

170 m2/g

surface coverage

surface coverage 3.2 μmol/m2

matrix

silica gel, spherical particle platform

matrix active group

C8 (octyl) phase

particle size

5 μm

pore size

120 Å

application(s)

food and beverages

separation technique

reversed phase

Quality Level

100

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General description

SUPELCOSIL LC-DB 针对碱性化合物的分析进行了特殊的去活化处理。与常规反相色谱柱相比，分析有机碱时该色谱柱保留时间更短、峰形更好和柱效更高。

Other Notes

探索最适于 HPLC 或 LC-MS 分析的 LiChropur 试剂

Legal Information

SUPELCOSIL is a trademark of Sigma-Aldrich Co. LLC

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
299295-04
299295-03
299295-02
299295-01
299294-04

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. What does DB stand for in Supelcosil LC-8DB HPLC column, Product 58354?

    DB stands for "deactivated base", and refers to the base treatment of the packing in this column. Without this treatment, basic compounds will adsorb to active acidic silanol sites.  If starting a new method, Supelco suggests using a new-technology/pure-silica C8, such as the Ascentis C8.

  4. Can the Supelcosil LC-8DB HPLC column, Product 58354, be used for acidic compounds?

    No, it is base treated, therefore acidic compounds will be adsorbed by the base treatment, resulting is peak tailing.  

  5. Do you have a cleaning procedure for Supelcosil LC-8DB HPLC column, Product 58354?

    Yes.

  6. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  7. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  8. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Vesna Cerkvenik-Flajs et al.
Analytica chimica acta, 663(2), 165-171 (2010-03-09)
An analytical method has been developed for the simultaneous determination of the following endectocide drugs in milk: ivermectin, abamectin, doramectin, moxidectin, eprinomectin, emamectin and nemadectin. Samples were extracted with acetonitrile, purified with solid-phase extraction on a reversed phase C(8), derivatised
J H Nichols et al.
Clinical chemistry, 40(7 Pt 1), 1312-1316 (1994-07-01)
This automated assay determines the concentration of the antidepressant fluoxetine (Prozac) and its active metabolite norfluoxetine in serum by reversed-phase HPLC with spectrophotometric detection. Extraction, injection, and quantification are performed by the Gilson Aspec automated sample handler. The patient's specimen
Jadwiga Piwowarska et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 805(1), 1-5 (2004-04-29)
A high-performance liquid chromatographic method is described for determination of lidocaine (2-(dietyloamino)-N-(2,6-dimetylofenylo) acetamid) and its metabolite, monoethylglycine xylidide (MEGX), in human serum containing various concentration of bilirubin. Lidocaine and its metabolite were extracted from human serum using dichloromethane. After separation
K Ary et al.
Journal of pharmaceutical and biomedical analysis, 26(2), 179-187 (2001-07-27)
A reversed-phase high-performance liquid chromatographic method with coulometric and UV detection has been developed for the simultaneous determination of morphine, morphine-3-glucuronide and morphine-6-glucuronide. The separation was carried out by using a Supelcosil LC-8 DB reversed-phase column and 0.1 M potassium
P K Kunicki
Journal of chromatography. B, Biomedical sciences and applications, 750(1), 41-49 (2001-02-24)
A HPLC-UV determination of clobazam and N-desmethylclobazam in human serum and urine is presented. After simple liquid-liquid extraction with dichloromethane the compounds and an internal standard diazepam were separated on a Supelcosil LC-8-DB column at ambient temperature under isocratic conditions
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