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66825-U

BIOshell™ A400 Protein C4 (3.4 μm) HPLC Columns

L × I.D. 10 cm × 2.1 mm, HPLC Column

别名:

Protein C4 Reversed-Phase Chromatography Column

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关于此项目

UNSPSC Code:
41115700
NACRES:
SB.52
L × i.d.:
10 cm × 2.1 mm
Particle size:
3.4 μm
Matrix active group:
C4 (butyl) phase
Pore size:
400 Å
Matrix:
spherical silica particle platform, superficially porous particle
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产品名称

BIOshell™ A400 Protein C4, 3.4 μm HPLC Column, 3.4 μm particle size, L × I.D. 10 cm × 2.1 mm

material

stainless steel hardware

Quality Level

100

agency

suitable for USP L26

description

Shell thickness (0.2 μm), Solid Core (3.0 μm)

product line

BIOshell™

feature

endcapped

manufacturer/tradename

BIOshell™

packaging

1 ea of

extent of labeling

0.4% carbon loading

parameter

600 bar max. pressure, 90 °C max. temp.

technique(s)

HPLC: suitable, LC/MS: suitable, UHPLC-MS: suitable, UHPLC: suitable

L × I.D.

10 cm × 2.1 mm

surface area

15 m2/g

surface coverage

4.2 μmol/m2

matrix

spherical silica particle platform, superficially porous particle

matrix active group

C4 (butyl) phase

particle size

3.4 μm

pore size

400 Å

operating pH range

2-9

separation technique

reversed phase

General description

BIOshell™ 400 Å Protein C4 columns contain 3.4 μm advanced Fused-Core particles with 400 Å pores that are specifically engineered to provide efficient separation of larger proteins. These columns provide excellent separations on very hydrophobic proteins such as those found in the cell membrane. The C4 ligand combined with an exhaustively endcapped surface ensures optimum protein recovery of larger or very hydrophobic biomolecules. The 3.4 μm Fused-Core particles allow a shorter analysis time yet similar back pressure to fully porous 1.7 μm, 300 Å fully porous C4 particles. These columns can be operated long term at pressures up to 9000 psi (600 bar), temperatures up to 90 °C, and within the pH range of 2 to 9.

Other Notes

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

Legal Information

BIOshell is a trademark of Sigma-Aldrich Co. LLC

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历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
USJUQ001677
USJUQ001676
USJUQ001675
USJUQ001674
USJUQ001673

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实验方案

BIOshell™ IgG 1000 Å C4 UHPLC Column for Improved Biomacromolecule Separations

BIOshell™ IgG 1000 Å C4 UHPLC Column for Improved Biomacromolecule Separations

HPLC Analysis of the Monoclonal Antibody (mAb) Erbitux (Cetuximab) on BIOshell A400 Protein C4

HPLC Analysis of the Monoclonal Antibody (mAb) Erbitux (Cetuximab) on BIOshell™ A400 Protein C4

Faster Protein LC (FP2LC)

Larger porous shell particles in BIOshell™ columns improve efficiency in U/HPLC analysis of peptides and proteins.

商品

BIOshell™ A400 Protein C4 U/HPLC Columns

BIOshell™ A400 Protein C4 columns contain 3.4 μm particles with 400 Å pores, which are derivatized with dimethylbutyl silane and exhaustively endcapped for optimum protein recovery.

Difluoroacetic Acid as an Effective Mobile Phase Modifier for the LC-UV/MS Analysis of Proteins

The article describes the use of difluoroacetic acid (DFA) as an effective alternative to acid modifiers, formic acid (FA) and trifluoroacetic acid (TFA) in the LC-UV/MS analysis of proteins.

HPLC Analysis of Protein Standards (1) on BIOshell™ A400 Protein C4

For use as a marker in SDS-PAGE; Albumin from chicken egg white, For use as a marker in SDS-PAGE; L-Lactic Dehydrogenase from rabbit muscle, Type XI, lyophilized powder, 600-1,200 units/mg protein

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相关内容

Large Molecule HPLC

High performance liquid chromatography (HPLC) can be used to separate and identify different large biomolecules such as protein and peptides in a sample. It is based on the pumping of a sample with a solvent (mobile phase) through a column packed with sorbent material (stationary phase) at a high pressure.

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货号GTIN
66825-U04061833388143