描述
HW-40F
产品线
TOYOPEARL®
表单
slurry
包装
bottle of 500 mL
参数
3 bar max. pressure
技术
HPLC: suitable
基质
hydroxylated methacrylic polymer
基质活性基团
hydroxylated methacrylate phase
粒径
30-60 μm
孔径
100-7000 Da MW range
工作pH值
2-13
分离技术
size exclusion (SEC)
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一般描述
Toyopearl HW-40 resin is a hydroxylated methacrylic polymer for size exclusion chromatography. It is typically used for high throughput desalting and buffer exchange. It also can remove surfactants such as Triton X100 from protein solutions. It is often used for smaller biomolecules with exclusion limits up to 10,000 Da.
应用
Toyopearl media fractionate mixtures of proteins and other large molecular weight compounds over a wide size range. Applications include separating RNA from protein, resolving oligosaccharides by degree of polymerization, and isolating agglutinin while maintaining a high hemagglutination titer.
特点和优势
Clean in place with 0.5 M NaOH or 0.1 M HCl.
外形
Shipped in 20% (v/v) ethanol.
法律信息
Toyopearl is a registered trademark of Tosoh Corporation
警示用语：
Warning
危险声明
危险分类
Flam. Liq. 3
WGK
WGK 1
个人防护装备
Eyeshields, Gloves, type N95 (US)
法规信息
新产品
此项目有
Francesca Magnani et al.
Nature protocols, 11(8), 1554-1571 (2016-07-29)
The thermostability of an integral membrane protein (MP) in detergent solution is a key parameter that dictates the likelihood of obtaining well-diffracting crystals that are suitable for structure determination. However, many mammalian MPs are too unstable for crystallization. We developed
M V Pokrovskaya et al.
Protein expression and purification, 82(1), 150-154 (2012-01-10)
We have cloned ansB (YPTB1411) gene from Yersinia pseudotuberculosis Q66CJ2 and constructed stable inducible expression system that overproduce L-asparaginase from Y. pseudotuberculosis (YpA) in Escherichiacoli BL21 (DE3) cells. For purification of YpA we used Q-Sepharose and DEAE-Toyopearl column chromatography. We
Shu-fen Xu et al.
Fitoterapia, 83(1), 153-160 (2011-11-01)
Persimmon proanthocyanidin was fractionated on Toyopearl TSK-HW-50-F to yield a fraction with strong inhibition on the catalytic activity and edema-inducing activity and lethality of Chinese cobra PLA(2). Thiolysis suggested that the terminal units included C, EGCG and myricetin, and epicatechin
Olga N Smol'kina et al.
Carbohydrate research, 347(1), 161-163 (2011-11-22)
A method is developed for the preparation of D-rhamnose from an O-polysaccharide (OPS) isolated by mild acid hydrolysis of Azospirillum brasilense SR75 cell mass. After the OPS hydrolysis, D-rhamnose was recovered by gel-permeation chromatography on Toyopearl TSK HW-40 and was
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