description
CM-650M
product line
TOYOPEARL®
form
slurry
feature
counter ion Na+
packaging
bottle of 250 mL
parameter
3 bar max. pressure
technique(s)
HPLC: suitable
matrix
methacrylate
matrix active group
carboxymethyl phase
particle size
65 μm
operating pH
5-10
capacity
0.10 meq/mL±0.02 meq/mL ion exchange capacity, 40 g/L±10 adsorption capacity (lysozyme), 50 mg/mL±10 mg/mL binding capacity (bovine haemoglobin)
compatibility
mode of use weak cation exchange chromatography
separation technique
cation exchange
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General description
Toyopearl CM-650 resin is a weak cation exchanger for biomolecule purification. It can be used for high throughput capture, intermediate purification, and polishing process steps.
Application
TOYOPEARL® CM-650M is used in separation media and ion exchange resins. TOYOPEARL® CM-650M has been used in cloning studies.
Features and Benefits
Clean in place with 0.5 M NaOH or 0.1 M HCl.
Physical form
Shipped in 20% (v/v) ethanol.
Legal Information
Toyopearl is a registered trademark of Tosoh Corporation
signalword
Warning
hcodes
Hazard Classifications
Flam. Liq. 3
wgk
WGK 1
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
新产品
此项目有
Ken-ichi Suzuki et al.
European journal of biochemistry, 270(4), 771-778 (2003-02-13)
A cellulase [endo-beta-1,4-D-glucanase (EC 3.2.1.4)] was isolated from the hepatopancreas of abalone Haliotis discus hannai by successive chromatographies on TOYOPEARL CM-650M, hydroxyapatite and Sephacryl S-200 HR. The molecular mass of the cellulase was estimated to be 66 000 Da by
Eri Shimizu et al.
Carbohydrate research, 338(24), 2841-2852 (2003-12-12)
An alginate lyase, termed HdAly in the present paper, was isolated from the hepatopancreas of abalone, Haliotis discus hannai, by ammonium sulfate fractionation, followed by TOYOPEARL CM-650M column chromatography. Enzymatic properties of HdAly were similar to those of previously reported
Shuuji Ootsuka et al.
Journal of biotechnology, 125(2), 269-280 (2006-04-20)
An endo-beta-1,4-mannanase was isolated from digestive fluid of Pacific abalone, Haliotis discus hannai, by successive chromatographies on TOYPEARL CM-650M, hydroxyapatite, and TOYOPEARL HW50F. The abalone mannanase, named HdMan in the present paper, showed a molecular mass of approximately 39,000 Da
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