description
DEAE-650C
product line
TOYOPEARL®
form
slurry
feature
counter ion Cl-
packaging
bottle of 250 mL
parameter
3 bar max. pressure
technique(s)
HPLC: suitable
matrix
methacrylate
matrix active group
DEAE (diethylaminoethyl) phase
particle size
100 μm
operating pH
2-10
capacity
0.10 meq/mL±0.02 meq/mL ion exchange capacity, 30 g/L±5 adsorption capacity (BSA), 30 mg/mL±5 mg/mL binding capacity (bovine serum albumin)
compatibility
mode of use weak anion exchange chromatography
separation technique
anion exchange
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General description
Toyopearl DEAE-650 resin is a weak anion exchanger for blood fractionation and other biomolecule purification. It can be used for high throughput capture, intermediate purification, and polishing process steps. If more powerful resolution is needed TSKgel DEAE-5PW resin should be used. Selectivity remains the same.
Application
TOYOPEARL® DEAE-650C is used in separation media and ion exchange resins. TOYOPEARL® DEAE-650C has been used in studies to develop supplements for immune enhancement.
Features and Benefits
Clean in place with 0.5 M NaOH or 0.1 M HCl.
Physical form
Shipped in 20% (v/v) ethanol.
Legal Information
Toyopearl is a registered trademark of Tosoh Corporation
signalword
Warning
hcodes
Hazard Classifications
Flam. Liq. 3
wgk
WGK 1
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
新产品
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H Lee et al.
Applied biochemistry and biotechnology, 97(2), 123-133 (2002-05-09)
A keratinolytic protease-producing microorganism was isolated from soybean paste waste and was identified as a strain of Bacillus sp. The keratinase was purified by polyethylene glycol precipitation and two successive column chromatographies with DEAE-Toyopearl 650C and Sephacryl S-200 HR. The
Hyug Chun et al.
Biological & pharmaceutical bulletin, 25(9), 1203-1208 (2002-09-17)
The purified polysaccharides from Piper nigrum were prepared as follows: a hot water extract of pepper seeds was fractionated by ultrafiltration with a 5-kDa-membrane cartridge. A fraction with 5 kDa or bigger molecules was successively purified by open column chromatography
H Chun et al.
Biological & pharmaceutical bulletin, 24(8), 941-946 (2001-08-21)
Polysaccharides are involved in biological responses and can activate complement system, which plays an important role in primary host defense mechanisms. We investigated anticomplementary activities from spice plants and selected thyme (Thymus vulgaris L.) as a potent complementary activator. Acidic
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