description
AF-Amino-650M
product line
TOYOPEARL®
form
liquid
packaging
bottle of 25 mL
manufacturer/tradename
Tosoh TOYOPEARL® AF, 08002
extent of labeling
100 μmol/mL±30 ligand density
parameter
3 bar max. pressure
technique(s)
HPLC: suitable
matrix
hydroxylated methacrylic polymer
matrix active group
amine-activated phase
particle size
65 μm
operating pH
2-13
separation technique
anion exchange
storage temp.
2-8°C
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General description
Toyopearl AF-Amino-650 resin is a reactive resin used for the coupling of specific ligands for affinity chromatography. Ligands are immobilized by either peptide bond formation or reductive amination through their respective carboxylate or aldehyde groups.
Application
TOYOPEARL® Affinity Media are used in affinity chromatography, protein chromatography and activated/functionalized matrices.
Physical form
Shipped in 20% (v/v) ethanol.
Legal Information
Toyopearl is a registered trademark of Tosoh Corporation
signalword
Warning
hcodes
Hazard Classifications
Flam. Liq. 3
wgk
WGK 1
ppe
Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter
法规信息
新产品
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S Knör et al.
Journal of thrombosis and haemostasis : JTH, 6(3), 470-477 (2008-01-16)
Hemophilia A is currently treated by infusions of the coagulation factor (F) VIII, of which production and purification remain a challenging task. Current purification procedures using immunoaffinity chromatography are cumbersome, expensive, and suffer from the instability of the applied antibody
S Yamauchi et al.
The Journal of biological chemistry, 274(4), 2456-2463 (1999-01-16)
Chondroitin 4-sulfotransferase, which transfers sulfate from 3'-phosphoadenosine 5'-phosphosulfate to position 4 of N-acetylgalactosamine in chondroitin, was purified 1900-fold to apparent homogeneity with 6.1% yield from the serum-free culture medium of rat chondrosarcoma cells by affinity chromatography on heparin-Sepharose CL-6B, Matrex
Zhenzhen Wen et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 879(20), 1732-1740 (2011-05-17)
In order to improve its stability, immobilized Concanavalin A (Con A) on Toyopearl adsorbents was conjugated with monomethoxy poly(ethylene glycol) succinimidyl propionate (mPEG-SPA) with different molecular weight. A colorimetric method using ninhydrin is proposed to determine the degree of PEGylation;
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