产品名称
TSKgel® Phenyl-5PW RP HPLC Column, phase phenyl, octadecyl, L × I.D. 7.5 cm × 8 mm, 10 μm particle size
material
glass
product line
TSKgel®
manufacturer/tradename
Tosoh 14007
extent of labeling
1 meq/mL ligand density (estimate)
parameter
no limitation salt concentration, 0-100% organic solvent, 2.5 mL/min flow rate, 20 bar max. pressure, 4-60 °C temperature
technique(s)
HPLC: suitable
L × I.D.
7.5 cm × 8 mm
matrix
hydroxylated methacrylic polymer
matrix active group
phenyl phase, octadecyl
particle size
10 μm
operating pH
2-12
separation technique
reversed phase
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General description
Polymer-based particles for reversed-phase (RP) HPLC generally have two benefits over silica-based particles: They are more tolerant of high and low pH operating conditions and they do not have the silanol interactions that cause peak tailing of strong basic analytes. Also, some silicas have an intrinsic metal content that may cause chelation problems with sensitive biomolecules, leading to poor recovery. Polymeric TSKgel® columns come in C18 and phenyl functional groups and a range of particle porosities. Fast kinetics and quantitative protein recovery at submicrogram loading are characteristic of nonporous resin-based TSKgel Octadecyl-NPR columns.
- Octadecyl-2PW particles have 125 A pores
- Octadecyl-4PW particles have 500 Apores
- Phenyl-5PW RP particles have 1000 A pores
- Octadecyl-NPR particles are nonporous
Physical form
Shipped in 30% methanol - 70% water.
Legal Information
TSKgel is a registered trademark of Tosoh Corporation
法规信息
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