description
AF-Tresyl-650M
product line
TOYOPEARL®
form
powder
packaging
bottle of 5 g
manufacturer/tradename
Tosoh TOYOPEARL® AF, 14471
extent of labeling
80 μmol/g dry resin ligand density
parameter
3 bar max. pressure
technique(s)
HPLC: suitable
matrix
hydroxylated methacrylic polymer particle platform
matrix active group
tresyl-activated phase
particle size
65 μm
pore size
1000 Å
separation technique
affinity
storage temp.
2-8°C
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General description
Toyopearl AF-Tresyl-650 resin is an activated resin which readily binds to amine and thiol groups. The resin is provided dry for introduction into a buffered solution containing protein or other ligand in neutral to slightly alkaline (pH 7-8) conditions.
Application
TOYOPEARL® Affinity Media are used in affinity chromatography, protein chromatography and activated/functionalized matrices.
Physical form
Shipped in dry, free flowing powder.
Legal Information
Toyopearl is a registered trademark of Tosoh Corporation
signalword
Warning
hcodes
Hazard Classifications
Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
target_organs
Respiratory system
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Gloves
法规信息
新产品
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S Knör et al.
Journal of thrombosis and haemostasis : JTH, 6(3), 470-477 (2008-01-16)
Hemophilia A is currently treated by infusions of the coagulation factor (F) VIII, of which production and purification remain a challenging task. Current purification procedures using immunoaffinity chromatography are cumbersome, expensive, and suffer from the instability of the applied antibody
S Yamauchi et al.
The Journal of biological chemistry, 274(4), 2456-2463 (1999-01-16)
Chondroitin 4-sulfotransferase, which transfers sulfate from 3'-phosphoadenosine 5'-phosphosulfate to position 4 of N-acetylgalactosamine in chondroitin, was purified 1900-fold to apparent homogeneity with 6.1% yield from the serum-free culture medium of rat chondrosarcoma cells by affinity chromatography on heparin-Sepharose CL-6B, Matrex
Zhenzhen Wen et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 879(20), 1732-1740 (2011-05-17)
In order to improve its stability, immobilized Concanavalin A (Con A) on Toyopearl adsorbents was conjugated with monomethoxy poly(ethylene glycol) succinimidyl propionate (mPEG-SPA) with different molecular weight. A colorimetric method using ninhydrin is proposed to determine the degree of PEGylation;
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