817500
TSKgel® Octadecyl-2PW (5 µm) HPLC Columns
L x I.D. 15 cm × 4.6 mm, C18 (octadecyl) phase
别名:
TSKgel® Reversed Phase HPLC Column (Polymer-based)
产品名称
TSKgel® Octadecyl-2PW HPLC Column, phase C18 (octadecyl), L × I.D. 15 cm × 4.6 mm, 5 μm particle size
物料
stainless steel hardware
产品线
TSKgel®
制造商/商品名称
Tosoh 17500
标记范围
1 meq/mL ligand density (estimate)
参数
0-100% organic solvent
1.2 mL/min flow rate
120 bar max. pressure
4-50 °C temperature
技术
HPLC: suitable
长度 × 内径
15 cm × 4.6 mm
基质
polymer particle platform
基质活性基团
C18 (octadecyl) phase
粒径
5 μm
孔径
125 Å
工作pH值
2-12
分离技术
reversed phase
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一般描述
Highly cross-linked polymethacrylate base material of TSKgel Octadecyl-2PW provides excellent stability in high pH buffer systems and can withstand rigorous cleaning with either acid or base. The 125Å pores allow for anaylsis of peptides up to 8,000 Da. Columns packed with larger particle sizes are available for semi-preparative scale separations.
Polymer-based particles for reversed-phase (RP) HPLC generally have two benefits over silica-based particles: They are more tolerant of high and low pH operating conditions and they do not have the silanol interactions that cause peak tailing of some basic analytes. Also, some silicas have an intrinsic metal content that may cause chelation problems with sensitive biomolecules, leading to poor recovery. Polymeric TSKgel columns come in C18 and phenyl functional groups and a range of particle porosities. Fast kinetics and quantitative protein recovery at submicrogram loading are characteristic of nonporous resin-based TSKgel Octadecyl-NPR columns.
- Octadecyl-2PW particles have 125Å pores
- Octadecyl-4PW particles have 500Å pores
- Phenyl-5PW RP particles have 1000Å pores
- Octadecyl-NPR particles are nonporous
外形
Shipped in 55% acetonitrile - 45% water.
法律信息
TSKgel is a registered trademark of Tosoh Corporation
法规信息
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