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UNSPSC Code:
41115711
eCl@ss:
32110501
NACRES:
NB.21
L × i.d.:
15 cm × 2 mm
Particle size:
7 μm
Matrix active group:
C18 (octadecyl) phase
Pore size:
500 Å
Matrix:
polymer particle platform
产品名称
TSKgel® Octadecyl-4PW HPLC Column, phase C18 (octadecyl), L × I.D. 15 cm × 2 mm, 7 μm particle size
material
stainless steel hardware
product line
TSKgel®
manufacturer/tradename
Tosoh 18755
extent of labeling
1 meq/mL ligand density (estimate)
parameter
no limitation salt concentration, 0-100% organic solvent, 0.22 mL/min flow rate, 100 bar max. pressure, 5-50 °C temperature
technique(s)
HPLC: suitable
L × I.D.
15 cm × 2 mm
matrix
polymer particle platform
matrix active group
C18 (octadecyl) phase
particle size
7 μm
pore size
500 Å
operating pH
2-12
separation technique
reversed phase
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General description
Highly cross-linked polymethacrylate base material of TSKgel Octadecyl-4PW provides excellent stability in high pH buffer systems and can withstand rigorous cleaning with either acid or base. The 500Å pore allows for anaylsis of proteins up to 200kDa while the particle size offerings allow for analytical, semi-preparative and preparative scale separations.
Polymer-based particles for reversed-phase (RP) HPLC generally have two benefits over silica-based particles: They are more tolerant of high and low pH operating conditions and they do not have the silanol interactions that cause peak tailing of some basic analytes. Also, some silicas have an intrinsic metal content that may cause chelation problems with sensitive biomolecules, leading to poor recovery. Polymeric TSKgel columns come in C18 and phenyl functional groups and a range of particle porosities. Fast kinetics and quantitative protein recovery at submicrogram loading are characteristic of nonporous resin-based TSKgel Octadecyl-NPR columns.
- Octadecyl-2PW particles have 125Å pores
- Octadecyl-4PW particles have 500Å pores
- Phenyl-5PW RP particles have 1000Å pores
- Octadecyl-NPR particles are nonporous
Physical form
Shipped in 60% methanol - 40% water.
Legal Information
TSKgel is a registered trademark of Tosoh Corporation
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