Product Name
尿素, Vetec™, reagent grade, 99%
等级
reagent grade
产品线
Vetec™
方案
99%
mp
132-135 °C (lit.)
密度
1.335 g/mL at 25 °C (lit.)
官能团
amine
SMILES字符串
NC(N)=O
InChI
1S/CH4N2O/c2-1(3)4/h(H4,2,3,4)
InChI key
XSQUKJJJFZCRTK-UHFFFAOYSA-N
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一般描述
尿素是一种离液剂，适用于蛋白质变性。它扰乱了蛋白质的二级、三级和四级结构中的氢键。尿素还可以干扰 DNA 二级结构中存在的氢键。
应用
用于蛋白质变性以及作为不溶性或变性蛋白的温和增溶剂。可用于从已由 6M 盐酸胍变性的样品(如包涵体)复性蛋白质。可与盐酸胍和二硫苏糖醇 (DTT) 一起用于复性变性蛋白以恢复其天然或活性形式。
法律信息
Vetec is a trademark of Merck KGaA, Darmstadt, Germany
储存分类代码
11 - Combustible Solids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
Sheehan D.
Physical Biochemistry: Principles and Applications (2013)
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Immortalized hepatocyte cell lines show only a weak resemblance to primary hepatocytes in terms of gene expression and function, limiting their value in predicting drug-induced liver injury (DILI). Furthermore, primary hepatocytes cultured on two-dimensional tissue culture plastic surfaces rapidly dedifferentiate
G L French et al.
Lancet (London, England), 2(8551), 117-119 (1987-07-18)
Tuberculostearic acid, a structural component of Mycobacterium tuberculosis, was identified by gas chromatography/mass spectrometry with selected ion monitoring in cerebrospinal fluid (CSF) from 13 patients with proven and 8 out of 9 patients with suspected tuberculous meningitis; the negative result
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