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Merck
CN

V900412

三(羟甲基)甲基甘氨酸

Vetec, reagent grade, ≥99%

别名:

N-三(羟甲基)甲基甘氨酸

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线性分子式:
(HOCH2)3CNHCH2CO2H
化学文摘社编号:
分子量:
179.17
EC Number:
227-193-6
UNSPSC Code:
12161700
PubChem Substance ID:
Beilstein/REAXYS Number:
1937804
MDL number:
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产品名称

三(羟甲基)甲基甘氨酸, Vetec, reagent grade, ≥99%

InChI key

SEQKRHFRPICQDD-UHFFFAOYSA-N

InChI

1S/C6H13NO5/c8-2-6(3-9,4-10)7-1-5(11)12/h7-10H,1-4H2,(H,11,12)

SMILES string

OCC(CO)(CO)NCC(O)=O

grade

reagent grade

product line

Vetec

assay

≥99%

form

crystalline powder

useful pH range

7.4-8.8

pKa (25 °C)

8.1

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Application

用于分离低分子量的肽的缓冲剂组分。

General description

三(羟甲基)甲基甘氨酸是最常用的电泳缓冲液,其负电荷比甘氨酸低。这反过来又有助于它更快地迁移。三(羟甲基)甲基甘氨酸也用于细胞团块的再悬浮。此外,它的高离子强度导致更多的离子运动和更少的蛋白质运动。这有助于在较低百分比的丙烯酰胺凝胶中分离低分子量蛋白质。与甘氨酸-十二烷基硫酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)系统相比,三(羟甲基)甲基甘氨酸也被用作拖尾离子,有助于在较低丙烯酰胺浓度下解析小蛋白质。

Legal Information

Vetec is a trademark of Merck KGaA, Darmstadt, Germany

存储类别

13 - Non Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


历史批次信息供参考:

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Encyclopedia of Dairy Sciences (2011)
Thierry Rabilloud
Journal of proteomics, 73(8), 1562-1572 (2010-04-17)
Electrophoretic separations of proteins are widely used in proteomic analyses, and rely heavily on SDS electrophoresis. This mode of separation is almost exclusively used when a single dimension separation is performed, and generally represents the second dimension of two-dimensional separations.
Christian Nilsson et al.
Electrophoresis, 31(3), 459-464 (2010-02-02)
Totally porous lipid-based liquid crystalline nanoparticles were used as pseudostationary phase for capillary electroseparation with LIF detection of proteins at physiological conditions using unmodified cyclic olefin copolymer capillaries (Topas, 6.7 cm effective length). In the absence of nanoparticles, i.e. in
Arpita Gantayet et al.
Biofouling, 29(1), 77-85 (2012-12-06)
The freshwater zebra mussel (Dreissena polymorpha) is a notorious biofouling organism. It adheres to a variety of substrata underwater by means of a proteinaceous structure called the byssus, which consists of a number of threads with adhesive plaques at the
Hermann Schägger
Nature protocols, 1(1), 16-22 (2007-04-05)
Tricine-SDS-PAGE is commonly used to separate proteins in the mass range 1-100 kDa. It is the preferred electrophoretic system for the resolution of proteins smaller than 30 kDa. The concentrations of acrylamide used in the gels are lower than in

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