General description
Excess transition metal ions, such as Cu(II), can generate hydroxyl radicals [OH•] in biological systems through Fenton-like reactions. Some antioxidants are able to chelate Cu(II) thereby inhibiting the formation of hydroxyl radicals and oxidative damage. The ZenBio Cupric Ion Chelating (CIC) Assay measures the capacity of test samples to chelate free cupric ions in solution thereby inhibiting Cu(II) binding to pyrocatechol violet (PV) which generates a highly colored complex. EDTA serves as a positive control capable of chelating cupric ions in a dose dependent manner. The CIC assay is an endpoint assay measuring absorbance of the Cu(II)-PV complex at λ = 632nm. CIC activity is determined as the percent of total PV / Cu(II) binding.
The AOX-16 kit contains everything required for performing the CIC assay using two 96-well plates including EDTA controls.