用于研究的螯合剂和还原剂
研究中的螯合剂
螯合剂可用作络合剂。作为缓冲成分、核酸/蛋白质降解抑制剂、酶反应催化剂、亲和层析和细胞培养剂，它们有助于各种化学和生化分析。螯合剂是平衡新陈代谢和相关疾病（如贫血、癌症、急性肾病、衰老、神经退行性疾病等）的有效治疗剂。在工业、分析和实验室实验中，这些金属离子螯合剂可用作萃取剂、指示剂和金属分离剂。
研究中的还原剂
还原剂可用于蛋白质纯化、变性和增溶等蛋白质组学方法，尤其擅长稳定蛋白质和肽中的游离巯基和还原二硫键（SH）。在蛋白质纯化过程中，还原剂可作为缓冲液中的酶稳定剂，防止半胱氨酸残基氧化造成的聚集。还原剂还可作为生物抗氧化剂，防止蛋白质氧化和核糖核酸酶变性。研究表明，将螯合试剂与还原剂结合使用有助于去除阳离子重金属和阴离子污染物（如砷）。
相关资源
- Article: Chelators
BioUltra Chelators. A chelate is a water-soluble complex between a metal ion and a complexing agent. It usually does not dissociate easily in solution, but forms an inert complex.
- Protocol: Nuclear Protein Extraction Without the Use of Detergent
Detergent-free procedure is recommended for nuclear protein preparation to avoid interference with labeling efficiency of extracted proteins.
- Calculators & Apps: Detergent-based Western Blot Stripping Buffer
This recipe for a detergent-based stripping solution enables the removal of antibodies from Western blots that have been developed with chemiluminescence or radioactive iodine or other isotopes.
- Flyer: Biochemicals for New and Growing Labs
Discover how our high-purity biological buffers, detergents, and reagents can help empower your innovation and build a better lab.
如要继续阅读，请登录或创建帐户。
暂无帐户？