Our portfolio of plateable Hepatocytes includes spheroid-qualified Hepatocytes for 3D cell culture applications. While our plateable and suspension Hepatocytes are ideal for 2D cell culture applications, our spheroid-qualified plateable Hepatocytes can provide more relevant models for applications such as enzyme induction, toxicity, drug screening, transporter efflux activity, potential drug-drug interactions, and disease modeling.
Our collection of spheroid-qualified plateable Hepatocyte is isolated from whole human livers and consists of a homogenous population of cells, with each donor providing documented consent for research use of non-transplantable organs or tissues. Every lot is guaranteed to contain a minimum of 4 million viable cells per vial, maintaining a post-thaw viability of ≥70%. These Hepatocytes undergo extensive characterization, encompassing mRNA induction data and enzyme profiling data. Additionally, they are validated to form 3D spheroids when cultured for a minimum of 7 days. In this protocol, we demonstrate how to thaw, plate, and form Hepatocyte spheroids from human spheroid-qualified plateable Hepatocytes.
This protocol was performed in a Class II laminar flow biohood and using an incubator set to 37°C and 5% CO2. Researchers should wear PPE such as a lab coat, safety glasses, and gloves.
Figure 1. Spheroid-qualified Hepatocytes cultured for various days on ULA surface. Cells were seeded at 4,000 cells per well into 96 well ULA plate. Images were taken, a) right after seeding and centrifugation, b) after 3 days of culturing, and c) after 8 days of culturing. All images were taken using Millicell® Digital Cell Imager at 10x magnification.
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