Ascentis^® Express HILIC U/HPLC Columns

HILIC chromatography is a variation of normal-phase chromatography with the distinction that one of the major components of the mobile phase is water. The high volatility of the mobile phase makes HILIC LC-MS friendly, and allows for increased MS sensitivity. Many classes of polar compounds can be retained on the Ascentis^® Express HILIC column including polar neutrals, polar acids, and polar and non-polar basic amines. The HILIC column provides orthogonal selectivity to the Ascentis^® Express C18, with the elution typically the opposite of that for C18. Non-polar compounds elute early and the highly polar compounds have greater retention and selectivity.

For high speed and high resolution separations on HPLC and UHPLC instruments, the  Fused-Core^_® particle technology of the Ascentis^® Express HILIC column means that efficiency is typically 40% higher in comparison to fully porous particulate columns with same particle size.

2.0 µm particles

Best Fused-Core UHPLC Column

An optimized solution for high-throughput small molecule analysis

2.7 µm particles

Fast HPLC on ANY System

A practical solution that delivers UHPLC performance from any HPLC

5.0 µm particles

The Lab Work-Horse Column

True plug and play solution for improving existing 3 or 5 µm fully porous particle HPLC columns