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MISSION® Application Data
We are committed to providing you with helpful application notes and data for the MISSION® TRC shRNA libraries. Below are links to data pieces and articles on various topics of interest. If you would like us to post your data (specific applications, validation of gene targets, etc.) on our site, please send us your experimental summary and data by e-mail.
Posters – MISSION® R&D
- A Screen of MISSION® shRNAs Targeting Tumor Suppressor Genes to Identify Factors Involved in A549 Paclitaxel Sensitivity
- Gene Silencing in Primary Astrocyte Cells using MISSION® TRC shRNA Lentiviral Particles (338 Kb PDF)
- Reproducible, Long-term Silencing in a High-throughput Lentiviral shRNA Screen (211 Kb PDF)
- Verification of RNA Interference at the Protein Level via Quantitative Proteomics Analysis (374 Kb PDF)
- Examination of Efficacy, Specificity, and Efficiency of an shRNA Lentiviral Library (357 Kb PDF)
- Efficient Gene Silencing with High Selectivity using MISSION® TRC shRNA Lentiviral Vectors
Posters – External Researchers
- Lentiviral shRNA Screen of Multidrug Resistant Associated Genes Identifies PRP-4 as a New Regulator of Chemoresistance in Human Ovarian Cancer (635 Kb PDF)
Zhenfeng Duan, M.D. Ph.D. – Harvard Medical School
- shRNA Phenotypic Screen To Identify Gene Targets Contributing to Androgen Independent Prostate Cancer Cell Growth (989 Kb PDF)
Daniel Gioeli Ph.D. – University of Virginia
- Mesenchymal Stem Cells: Cell Death or Survival? Interaction with Other Stress Pathways (887 Kb PDF)
by Oliver L. and Vallette FM, Center for Cancer Research Nantes - Angers INSERM UMR 892, University of Nantes, France
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Publications
1.
Duan Z, Weinstein EJ, Ji D, Ames RY, Choy E, Mankin H, Hornicek FJ. 2008. Lentiviral short hairpin RNA screen of genes associated with multidrug resistance identifies PRP-4 as a new regulator of chemoresistance in human ovarian cancer. Mol Cancer Ther. 7(8):2377-2385. https://doi.org/10.1158/1535-7163.mct-08-0316
2.
Bauer JA, Ye F, Marshall CB, Lehmann BD, Pendleton CS, Shyr Y, Arteaga CL, Pietenpol JA. 2010. RNA interference (RNAi) screening approach identifies agents that enhance paclitaxel activity in breast cancer cells. Breast Cancer Res. 12(3): https://doi.org/10.1186/bcr2595
3.
Moffat J, Grueneberg DA, Yang X, Kim SY, Kloepfer AM, Hinkle G, Piqani B, Eisenhaure TM, Luo B, Grenier JK, et al. 2006. A Lentiviral RNAi Library for Human and Mouse Genes Applied to an Arrayed Viral High-Content Screen. Cell. 124(6):1283-1298. https://doi.org/10.1016/j.cell.2006.01.040
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