产品名称
抗酪氨酸羟化酶抗体, Chemicon®, from rabbit
antibody product type
primary antibodies
biological source
rabbit
antibody form
affinity isolated antibody
clone
polyclonal
purified by
affinity chromatography
species reactivity
human, mollusk, feline, ferret, Aplysia (marine mollusc), rat, squid, Drosophila, mouse
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable
immunofluorescence: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
immunoprecipitation (IP): suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Quality Level
Analysis Note
阳性对照:脑(纹状体,交感神经末梢)和肾上腺。
阴性对照:肝脏。
蛋白质印迹分析:
利用该批次的1:1000稀释液检测10 μg PC12裂解液中的酪氨酸羟化酶。
Application
前一批抗体采用1:100-1:1000稀释度,4°C条件,持续1-3天。通过间接免疫荧光和免疫过氧化物酶而对新鲜冷冻和石蜡包埋的组织样品进行染色。
免疫荧光:
前一批抗体采用1:100-1:1000稀释度。
免疫沉淀
前一批抗体用于免疫沉淀。ELISA:
前一批抗体用于ELISA。终端用户需自行确定最佳的稀释度。
神经递质 & 受体
神经元 & 胶质标记
神经科学
Biochem/physiol Actions
Disclaimer
General description
Immunogen
Physical form
Preparation Note
Legal Information
未找到合适的产品?
试试我们的产品选型工具.
存储类别
10 - Combustible liquids
wgk
WGK 1
商品
Antibodies combine with specific antigens to generate an exclusive antibody-antigen complex. Learn about the nature of this bond and its use as a molecular tag for research.
Learn differences in monoclonal vs polyclonal antibodies including how antibodies are generated, clone numbers, and antibody formats.
Immunofluorescence uses antibody-conjugated fluorescent molecules for protein localization, modification confirmation, and protein complex visualization.
Explore the basics of working with antibodies including technical information on structure, classes, and normal immunoglobulin ranges.
实验方案
Tips and troubleshooting for FFPE and frozen tissue immunohistochemistry (IHC) protocols using both brightfield analysis of chromogenic detection and fluorescent microscopy.
相关内容
抗体的质量决定了整个免疫检测的成败, 是在选择抗体时首选的考虑因素。基于Sigma®、Chemicon®、Upstate®、Mil l ipore®、 Calbiochem®和Novagen®等子品牌的强强联合,默克在创新的 免疫原设计、免疫、挑选、筛选和验证等方面有着数十年的经验, 创造出许多被广泛使用的抗体,如4G10,Neun,组蛋白修饰等抗 体都是相关领域的“金标”抗体。 我们的技术和科研背景使默克成为高质量抗体的主要设计者和生产 者,而不仅仅是经销商。毋庸置疑,我们的每个小瓶中的抗体都包 含着大量的科学研究成果。
Find protein research tools to prepare, isolate, and analyze proteins. Organized by how to extract, protect, purify, enrich, modify, and quantify proteins.
我们的科学家团队拥有各种研究领域经验,包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系客户支持