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ISEQ00010

Immobilon® -PSQ PVDF Membrane

1 roll, 27 cm x 3.75 m, 0.2 µm pore size, transfer membrane for Western blotting

Synonym(s):

Western blotting membrane, blotting membrane, transfer membrane

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About This Item

UNSPSC Code:
41105339
eCl@ss:
32031602
NACRES:
NB.23
Material:
PVDF membrane, plain filter, white filter
Detection method:
chemiluminescent, colorimetric, radioactive
Pore size:
0.2 μm pore size
Compatibility:
for use with Amido black, for use with CPTS, for use with Colloidal gold, for use with Coomassie brilliant blue, for use with India ink, for use with Ponceau-S red, for use with Sypro<TMSYMBOL></TMSYMBOL> ruby, for use with Toluidine blue, for use with Transillumination
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Product Name

Immobilon®-PSQ PVDF Membrane, 1 roll, 27 cm x 3.75 m, 0.2 µm pore size, Hydrophobic PVDF Transfer Membrane for western blotting.

Quality Level

400

material

PVDF membrane, plain filter, white filter

feature

hydrophobic

manufacturer/tradename

Immobilon®

technique(s)

dot blot: suitable, western blot: suitable

filter L × W

27 cm × 3.75 m

pore size

0.2 μm pore size

capacity

262 μg/cm2 adsorption capacity (insulin), 340 μg/cm2 adsorption capacity (BSA), 448 μg/cm2 adsorption capacity (goat IgG)

compatibility

for use with Amido black, for use with CPTS, for use with Colloidal gold, for use with Coomassie brilliant blue, for use with India ink, for use with Ponceau-S red, for use with Sypro<TMSYMBOL></TMSYMBOL> ruby, for use with Toluidine blue, for use with Transillumination

detection method

chemiluminescent, colorimetric, radioactive

shipped in

ambient

General description

The Immobilon®-PSQ membrane is a 0.2 μm microporous membrane made up of polyvinylidene fluoride (PVDF). It has been developed to maximize protein binding. Immobilon®-PSQ membrane is recommended for direct protein sequencing and immunodetection following electroblotting of protein from electrophoresis gels (or dot binding of purified protein samples), especially for proteins with molecular weights less than 20,000. This membrane is compatible with tank and semi-dry electroblotting systems.

Application

Immobilon®-PSQ PVDF Membrane has been used in western blot.

Features and Benefits

Immobilon®-PSQ Membrane:
  • 0.2 μm pore size and a large internal structure
  • Higher protein adsorption and sequencing yields than other membranes
  • Prevents blow-through of low molecular weight proteins

Legal Information

Immobilon is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Protocols

Cell Lysis and Protein Extraction for Western Blotting

Protocol for sample preparation for cell lysis and efficient protein extraction from cultured tissues and cells for subsequent Western blotting.

Stripping and Reprobing Western Blotting Membranes

This page shows and discusses three protocols for stripping and reprobing a western blot membrane.

WB检测样本前处理：细胞裂解和蛋白提取

Western blotting前用于培养组织和细胞的细胞裂解和有效蛋白提取的样品制备实验方案。

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Global Trade Item Number

SKUGTIN
ISEQ0001004053252334306