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WBKLS

Immobilon Western Chemiluminescent HRP Substrate

Immobilon Western HRP Substrate provides high sensitivity for Chemiluminescent detection in western or dot/slot/spot blotting applications on both PVDF and nitrocellulose membranes, and is compatible with all commonly used buffers and blocking reagents.

Synonym(s):

HRP substrate, chemiluminescent detection substrate, detection substrate

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About This Item

UNSPSC Code:
41116010
NACRES:
NB.22
eCl@ss:
42029053
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conjugate

peroxidase conjugate

Quality Level

200

packaging

pkg of 250 mL Luminol, pkg of 250 mL Peroxide solution

manufacturer/tradename

Immobilon®

technique(s)

western blot: suitable

membrane area

5000 cm2 , membrane coverage

detection method

chemiluminescent

shipped in

ambient

General description

Immobilon Western HRP Substrate is compatible with all commonly used buffers and blocking reagents and provides high sensitivity for chemiluminescent detection in western or dot/slot/spot blotting applications on both PVDF and nitrocellulose membranes.

Application

Immobilon Western Chemiluminescent HRP Substrate is suitable for western blotting.

Legal Information

Immobilon is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

12 - Non Combustible Liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

wgk

WGK 2

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Protocols

Cell Lysis and Protein Extraction for Western Blotting

Protocol for sample preparation for cell lysis and efficient protein extraction from cultured tissues and cells for subsequent Western blotting.

Stripping and Reprobing Western Blotting Membranes

This page shows and discusses three protocols for stripping and reprobing a western blot membrane.

WB检测样本前处理：细胞裂解和蛋白提取

Western blotting前用于培养组织和细胞的细胞裂解和有效蛋白提取的样品制备实验方案。

View All Protocols

Articles

Dot/Slot (Filtration) Blotting

The possible causes and potential remedies for challenges encountered dot/slot blotting, which uses vacuum filtration to transfer protein onto a microporous membrane.

Western Blotting: Why Are Observed and Calculated Molecular Weights Different?

A discussion of factors affecting observed vs. calculated molecular weight in gel electrophoresis and western blotting.

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Global Trade Item Number

SKUGTIN
WBKLS005008436037125140
WBKLS050004053252616754
WBKLS010008436037125102