产品名称
Immobilon Western 化学发光 HRP 底物, Immobilon Western HRP Substrate provides high sensitivity for Chemiluminescent detection in western or dot/slot/spot blotting applications on both PVDF and nitrocellulose membranes, and is compatible with all commonly used buffers and blocking reagents.
conjugate
peroxidase conjugate
packaging
pkg of 250 mL Luminol
pkg of 250 mL Peroxide solution
manufacturer/tradename
Immobilon®
technique(s)
western blot: suitable
membrane area
5000 cm2 , membrane coverage
detection method
chemiluminescent
shipped in
ambient
Quality Level
Application
Disclaimer
General description
Legal Information
存储类别
12 - Non Combustible Liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
商品
The possible causes and potential remedies for challenges encountered dot/slot blotting, which uses vacuum filtration to transfer protein onto a microporous membrane.
A discussion of factors affecting observed vs. calculated molecular weight in gel electrophoresis and western blotting.
实验方案
Protocol for sample preparation for cell lysis and efficient protein extraction from cultured tissues and cells for subsequent Western blotting.
This page shows and discusses three protocols for stripping and reprobing a western blot membrane.
Western blotting前用于培养组织和细胞的细胞裂解和有效蛋白提取的样品制备实验方案。
本页内容介绍和讨论了三种免疫印迹膜剥离和重新检测的方案。
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