MCF7 Cell Line human
NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 86012803, human breast (adenocarcinoma), Epithelial-like
别名:
IBMF-7 Cells, MCF7 Cells, Michigan Cancer Foundation-7 Cells
生物来源
human breast (adenocarcinoma)
包装
tube of 5 μg 86012803-DNA-5UG
pkg of vial of cells 86012803-1VL
生长模式
Adherent
核型
2n = 46, hypertriploid to hypotetraploid
形态学
Epithelial-like
产品
Not specified
受体
Reported to express the oestrogen and progesterone receptor
技术
cell culture | mammalian: suitable
相关疾病
cancer
运输
dry ice
储存温度
−196°C
应用
Tumourigenicity and virus studies: Type B and C viruses
生化/生理作用
Established from the pleural effusion from a 69 year female caucasian suffering from a breast adenocarcinoma. Cells exhibit some features of differentiated mammary epithelium including oestradiol synthesis and formation of domes. Cells may carry B or C type retrovirus and are considered to represent a category 2 pathogen (P2 containment). Cells express both the wild type and variant oestrogen receptors as well as progesterone receptor. The cells should be handled under laboratory containment level 2.
Human Caucasian breast adenocarcinoma
STR-PCR Data:
Amelogenin: X
CSF1PO: 10
D13S317: 11
D16S539: 11,12
D5S818: 11,12
D7S820: 8,9
THO1: 6
TPOX: 9,12
vWA: 14,15
Amelogenin: X
CSF1PO: 10
D13S317: 11
D16S539: 11,12
D5S818: 11,12
D7S820: 8,9
THO1: 6
TPOX: 9,12
vWA: 14,15
制备说明
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids NEAA + 10% Foetal Bovine Serum FBS / FCS.
Split sub-confluent cultures (70-80%) 1:2 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% Trypsin or Trypsin/EDTA; 5% CO2; 37°C.
其他说明
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