D4527
脱氧核糖核酸酶 I 来源于牛胰腺
Type II, lyophilized powder, Protein ≥80 %, ≥2,000 units/mg protein
别名:
DNase I, 脱氧核糖核酸 5′-寡核苷酸-水解酶
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化学文摘社编号:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-667-0
MDL number:
Specific activity:
≥2,000 units/mg protein
Biological source:
bovine pancreas
biological source
bovine pancreas
type
Type II
form
lyophilized powder
specific activity
≥2,000 units/mg protein
mol wt
~31 kDa
purified by
chromatography
composition
Protein, ≥80%
technique(s)
DNA extraction: suitable
solubility
0.15 M NaCl: soluble 5.0 mg/mL, clear
suitability
suitable for molecular biology
application(s)
diagnostic assay manufacturing
diagnostic assay manufacturing
foreign activity
Chymotrypsin ≤0.01%, Protease ≤0.005%, RNase ≤0.01%
shipped in
wet ice
storage temp.
−20°C
Quality Level
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Application
DNAse I可用于切开DNA，作为将标记碱基插入DNA的第一步。来自Sigma的酶已在Madin-Darby犬肾（MDCK）II细胞系的细胞裂解物制备中与RNAse一起用于核酸酶的储备。它也用于从辛德比斯病毒中提取RNA。
来自牛胰腺的脱氧核糖核酸酶I已用于两种类似于脱氧核糖核酸酶I的新型人类基因的鉴定、定位和表达。来自牛胰腺的脱氧核糖核酸酶I也已用于研究可从牛胰腺中获得高活性RNase、DNase、胆固醇酯酶和胰蛋白酶的新方法。
用于从蛋白质样品中除去 DNA。
Biochem/physiol Actions
DNase I是一种内切核酸酶，可作用于与嘧啶相邻的磷酸二酯键以产生具有末端5′-磷酸的聚核苷酸。当Mg2+存在时，DNAse I可独立切割DNA的每条链且切割位点是随机的。当Mn2+存在时，两条DNA链都几乎是在同样的位置被切割。最适pH在7-8之间。二价阳离子如Mn2+, Ca2+, Co2+以及Zn2+都是酶的激活剂。5 mM浓度的Ca2+可稳定酶免收蛋白水解酶的消化。来自牛胰腺的DNAse I由四种色谱可区分的组分A，B，C和D组成，它们的摩尔比分别为4:1:1。仅有少量的D被发现。2-巯基乙醇、螯合剂、十二烷基硫酸钠（SDS）和肌动蛋白都已知可抑制酶的活性。
Features and Benefits
有效 切割DNA及从蛋白质样品中去除DNA
Physical form
含有氯化钙
Preparation Note
酶粉可使用水或除磷酸盐缓冲液外任何pH 4.0-9.0的缓冲液进行重悬。应避免钙螯合剂。溶液0.15 M NaCl的10 mg/mL DNAse I溶液分装保存在−20 °C一周后可能会丢失<10%的活性。同样的溶液保存在2-8 °C则可能丢失约20%的活性。当在pH 5和7之间时，它可在60 °C维持活性最长达五小时，并在68 °C <10分钟内丢失活性。它在乙酸缓冲液（pH 5.0）和tris缓冲液((pH 7.2)，1 mg/mL浓度)中以6%/小时的速率丢失活性。
Analysis Note
蛋白由双缩脲法测定。
Other Notes
一Kunitz单位的酶在以I型或III型DNA作为底物时，在 pH 5.0，25°C的条件下在每ml每分钟内可引起0.001的A260变化。
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
动植物源性产品
低风险生物材料
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实验方案
To standardize a procedure for the enzymatic assay of Deoxyribonuclease I.
建立脱氧核糖核酸酶I酶促测定的标准化操作流程。
相关内容
QC Methods
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