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DUO92104

Duolink® In Situ Orange Starter Kit Mouse/Goat

别名:

in situ Proximity Ligation Assay reagent, Protein Protein Interaction Kit

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关于此项目

NACRES:
NA.32
UNSPSC Code:
41116158
Fluorescence:
λex 554 nm; λem 576 nm (orange) (Cyanine 3; Zeiss Filter set 20)
Technique(s):
proximity ligation assay: suitable
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product line

Duolink®

Quality Level

100

technique(s)

proximity ligation assay: suitable

fluorescence

λex 554 nm; λem 576 nm (orange) (Cyanine 3; Zeiss Filter set 20)

suitability

suitable for fluorescence

Application

Duolink®proximity ligation assay(PLA®) allows for endogenous detection of protein interactions, post translational modifications, and protein expression levels at the single molecule level in fixed cells and tissue samples.

Follow the Duolink® In Situ Fluorescence Protocol to use this product. A set of short instructionsis also available.

Visit our Duolink® PLA Resource Center for information on how to run a Duolink® experiment, applications, troubleshooting, and more.

To perform a complete Duolink® PLA in situ experiment you will need two primary antibodies (PLA, IHC, ICC or IF validated) that recognize two target epitopes. This starter kit supplies all other necessary reagents for 30 Duolink® PLA reactions, which include a pair of PLA probes (Anti-Mouse PLUS and Anti-Goat MINUS), orange detection reagents, wash buffers, and mounting medium.Note that the primary antibodies must come from the same species as the Duolink® PLA probes. Analysis is carried out using standard immunofluorescence assay equipment.
Specificity
The Duolink® In Situ Orange Starter Kit Mouse/Goat requires one primary antibody from mouse and one primary antibody from goat. Orange fluorescence detection reagents are often used with Cyanine 3 filter.

Application Note
Two primary antibodies raised in different species are needed. Test your primary antibodies (IgG-class, mono- or polyclonal) in a standard immunofluorescence (IF), immunohistochemistry (IHC) or immunocytochemistry (ICC) assay to determine the optimal fixation, blocking, and titer conditions. Duolink® PLA in situ reagents are suitable for use on fixed cells, cytospin cells, cells grown on slide, formalin-fixed, paraffin embedded (FFPE), or tissue (fresh or frozen). No minimum number of cells is required.

Let us do the work for you, learn more about our Custom Service Program to accelerate your Duolink® projects

View full Duolink® product list

Features and Benefits

  • No overexpression or genetic manipulation required
  • High specificity (fewer false positives)
  • Single molecule sensitivity due to rolling circle amplification
  • Relative quantification possible
  • No special equipment needed
  • Quicker and simpler than FRET
  • Increased accuracy compared to co-IP
  • Publication-ready results

Legal Information

Duolink is a registered trademark of Merck KGaA, Darmstadt, Germany
PLA is a registered trademark of Merck KGaA, Darmstadt, Germany

试剂盒组分也可单独购买

产品编号
说明
化学品安全说明书 & 价格
  • DUO92006
    Duolink® In Situ PLA® Probe Anti-Goat MINUS, Affinity purified Donkey anti-Goat IgG (H+L)
    化学品安全说明书
  • DUO92001
    Duolink® In Situ PLA® Probe Anti-Mouse PLUS
    化学品安全说明书
  • DUO92007
    Duolink® In Situ Detection Reagents Orange
    化学品安全说明书
  • DUO82049
    Duolink® In Situ Wash Buffers, Fluorescence
    化学品安全说明书
  • DUO82040
    Duolink® In Situ Mounting Medium with DAPI
    化学品安全说明书

signalword

Danger

flash_point_f

Not applicable

flash_point_c

Not applicable

wgk

WGK 3

Hazard Classifications

Aquatic Chronic 2 - ED ENV 1 - Met. Corr. 1 - Resp. Sens. 1 - Skin Sens. 1

存储类别

10 - Combustible liquids

法规信息

低风险生物材料
常规特殊物品

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实验方案

Duolink® PLA Fluorescence Protocol

This protocol describes how to perform immunofluorescent detection of proteins in cells and tissue.

Duolink® In Situ Short Instructions - Fluorescence

This page details the Duolink® In Situ Short Protocol for fluorescence detection

Duolink® 原位精简操作指南 - 荧光

本页详细介绍了Duolink®原位精简实验方案

商品

Duolink® PLA Technology for Neuroscience Applications

Duolink® proximity ligation assay used to study neuron interactions furthering neuroscience research.

Duolink® PLA Troubleshooting Guide

Support information including tips and tricks, frequently asked questions, and basic troubleshooting.

How to Optimize the Duolink® Proximity Ligation Assay

Things to consider for preparation, setup and execution of the Duolink® assay protocol

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全球贸易项目编号

货号GTIN
DUO92104-1KT04061833592229