E3138
抗-表皮生长因子受体单克隆抗体 小鼠抗
clone F4, ascites fluid
别名:
Anti-EGFR
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关于此项目
Conjugate:
unconjugated
Clone:
F4, monoclonal
Application:
ARR, ELISA (i), IHC (f), IHC (p), IP, WB
Citations:
28
biological source
mouse
conjugate
unconjugated
antibody form
ascites fluid
antibody product type
primary antibodies
clone
F4, monoclonal
contains
15 mM sodium azide
species reactivity
human
packaging
antibody small pack of 25 μL
technique(s)
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, immunohistochemistry (frozen sections): suitable, immunoprecipitation (IP): suitable, indirect ELISA: suitable, microarray: suitable, western blot: 1:2,000
isotype
IgG1
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... EGFR(1956)
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General description
Monoclonal Anti-Epidermal Growth Factor (EGF) Receptor (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. The receptor for epidermal growth factor is an integral cell membrane protein of 170 kD, which spans the membranes of a wide range of normal and malignant epithelial cells. EGF-R have been identified in sarcomas, gliomas, breast, bladder, lung and gynecological tumors. The EGF-R has a half-life of approximately 10 hours in human fibroblasts, but in the presence of EGF this value is reduced to about 1 hour.
Immunogen
synthetically produced residues 985-996 of the human EGF receptor.
Application
Monoclonal Anti-Epidermal Growth Factor Receptor antibody produced in mouse has been used in:
- western blotting
- immunoprecipitation
- immunocytochemistry
- immunohistochemical staining
Biochem/physiol Actions
EGFR is a transmembrane glycoprotein that binds to the EGF family. It is a pivotal regulator of cell proliferation, growth, differentiation and DNA repair after radiation-induced damage. Mutations in the gene encoding EGFR are associated with a number of malignancies including lung cancer and colorectal cancer.
Epidermal growth factor receptor (EGF-R) is a tyrosine-specific protein kinase with the capacity to phosphorylate tyrosine residues located near its carboxy-terminus.
This antibody recognizes specifically an intracellular domain of the EGF receptor.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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存储类别
10 - Combustible liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
动植物来源生物产品
常规特殊物品
此项目有
Changes in expression of epidermal growth factor receptors by anterior pituitary cells during the estrous cycle: cyclic expression by gonadotropes
Armstrong J and Childs GV
Endocrinology, 138(5), 1903-1908 (1997)
Localization of the epidermal growth factor (EGF) and epidermal growth factor receptor (EGFR) in the bovine testis
Kassab M, et al.
Journal of Molecular Histology, 38(3), 207-214 (2007)
Yuen-Keng Ng et al.
Melanoma research, 24(3), 207-218 (2014-04-09)
The BRAF inhibitor vemurafenib is currently used for treating patients with BRAF V600E mutant melanoma. However, the responses to vemurafenib are generally partial and of relatively short duration. Recent evidence suggests that activation of the epidermal growth factor receptor (EGFR)/erbB
T Cerny et al.
British journal of cancer, 54(2), 265-269 (1986-08-01)
Epidermal growth factor receptor (EGF-R) expression was assessed in 63 lung tumour samples with a monoclonal antibody (EGF-R1) by indirect immunoperoxidase staining on cryostat sections. All 15 small cell lung cancer samples were negative whereas over 80% of the 48
Tyrosine phosphorylation mapping of the epidermal growth factor receptor signaling pathway
Steen H, et al.
The Journal of Biological Chemistry, 277(2), 1031-1039 (2002)
相关内容
View an automated protocol for Duolink® assays on the AAW™ automated assay workstation and see results comparing manual vs automated runs.
在 AAW™ 自动测试工作站上查看 Duolink® 检测的自动协议，并查看手动与自动运行的结果比较。
Datasheet
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