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F8687

Fetal Bovine Serum

Australia origin, γ-irradiated, sterile-filtered, suitable for cell culture, suitable for hybridoma

Synonym(s):

Fetal Bovine Serum, FBS, Fetal Bovine Sera, GI FBS, GI FCS, GI Fetal Bovine Sera, GI Fetal Bovine Serum, FCS, sera, serum

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About This Item

UNSPSC Code:
12352207
MDL number:
MFCD00132239
Biological source:
bovine fetus
Origin:
Australia origin
Sterility:
γ-irradiated, sterile-filtered
Shipped in:
dry ice
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biological source

bovine fetus

Quality Level

500

sterility

γ-irradiated, sterile-filtered

product line

FBS Classic

composition

hemoglobin, ≤0.18 mg/mL

origin

Australia origin

technique(s)

cell culture | hybridoma: suitable, cell culture | mammalian: suitable

impurities

≤5 EU/mL endotoxin

shipped in

dry ice

storage temp.

−20°C

Application

Fetal Bovine Serum has been used in the Roswell Park memorial institute medium-1640 (RPMI-1640) medium to culture myeloma cell lines, RPMI-8226 and U-266. It has also been used as a supplement in Dulbecco′s modified Eagle medium (DMEM) to culture human umbilical vein endothelial cells (HUVECs) and 293 T cells.

Biochem/physiol Actions

Fetal Bovine Serum (FBS) is the commonly used serum to supplement cell culture media due to its high nutritional content. FBS, despite its low protein content, is effective in promoting and maintaining the growth of vertebrate mammalian and insect cells. It can be used as a supplement to culture embryonic stem (ES) cells. FBS can also be used to prepare feeder layers and conditioned media.

Analysis Note

  • Endotoxin and hemoglobin tested

Other Notes

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Storage Class

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
WXBF5048V
WXBF3346V
WXBD9363V
WXBD5226V
WXBD7799V

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Articles

Fetal Bovine Serum (FBS) – What You Need to Know

Review the basics of using fetal bovine (or calf) serum for cell culture and how sourcing and regulations affect the price of sera for cell-based assays.

胎牛血清 (FBS) – 基础知识

回顾使用胎牛（或小牛）血清进行细胞培养的基础知识，以及采购和法规如何影响细胞测定所用血清的价格。

高品质澳大利亚胎牛血清和小牛血清现货供应

默克提供血清产品，为您提供高品质澳大利亚胎牛血清和小牛血清现货和解决方案。牛血清由于包含丰富的营养成分，通常被添加到细胞培养液中，用于促进和维持脊椎动物，牛血清的来源地很多，其中澳大利亚来源的牛血清被公认为是品质更优。

Yan Zhang et al.
Artificial cells, nanomedicine, and biotechnology, 48(1), 60-67 (2019-12-20)
Background: Colon cancer is the most frequently lethal cancer in digestive system. Herein, we tested the influences of UNC5B antisense lncRNA 1 (UNC5B-AS1) on colon cancer cell growth and metastasis, along with the regulatory function of UNC5B-AS1 in microRNA-622 (miR-622)
Isolation and maintenance of avian ES cells
Petitte JN
Handbook of Stem Cells, 471-477 (2004)
Peng Zhang et al.
International journal of stem cells, 14(2), 229-239 (2021-02-27)
Implantation of bone marrow-derived mesenchymal stem cells (BMSCs) has been recognized as an effective therapy for attenuating acute lung injury (ALI). This study aims to discover microRNA (miRNA)-mediated improvement of BMSCs-based therapeutic effects. Mice were treated with lipopolysaccharide (LPS) for
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