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Merck
CN

M7292

M16 培养基

With sodium bicarbonate and lactic acid, without penicillin and streptomycin, liquid, sterile-filtered

别名:

M16培养基溶液, 细胞培养基, 细胞培养基M16

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关于此项目

NACRES:
NA.75
UNSPSC Code:
12352207
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产品名称

M16 培养基, With sodium bicarbonate and lactic acid, without penicillin and streptomycin, liquid, sterile-filtered

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | embryo: suitable

impurities

endotoxin, tested

components

NaHCO3: 2.101 g/L
phenol red: 0.0106 g/L
sodium pyruvate: 0.0363 g/L
glucose: 1.0 g/L (Dextro)

shipped in

ambient

storage temp.

2-8°C

Quality Level

Analysis Note

溶液中的沉淀不影响本品性能。
该产品经过测试,能够支持单细胞小鼠胚胎发育成扩大的胚泡。对胚泡发育的最低要求是 80%。

Application

M2 和 M16 培养基是植入前期胚胎体外培养的常用培养基。它是一种改良的 Krebs-Ringer 碳酸氢盐溶液,与 Whitten′s 培养基非常相似。M16 含有丙酮酸和乳酸作为能量来源,因为植入前胚胎不能有效利用葡萄糖。

Preparation Note

添加 0.06g/L 青霉素 G 钾盐和 0.05g/L 硫酸链霉素。

存储类别

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

法规信息

动植物来源培养基
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历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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Qian-Qian Sha et al.
Nature communications, 9(1), 3477-3477 (2018-08-30)
Trimethylation of histone H3 on lysine-4 (H3K4me3) is associated with gene-regulatory elements, but its transcription-independent function in cell division is unclear. CxxC-finger protein-1 (CFP1) is a major mediator of H3K4 trimethylation in mouse oocytes. Here we report that oocyte-specific knockout
Marco Ragusa et al.
RNA biology, 16(9), 1237-1248 (2019-05-29)
Circular RNAs (circRNAs) have a critical role in the control of gene expression. Their function in spermatozoa (SPZ) is unknown to date. Twenty-eight genes, involved in SPZ/testicular and epididymal physiology, were given in circBase database to find which of them
Hong-Bin Liu et al.
Advanced science (Weinheim, Baden-Wurttemberg, Germany), 6(15), 1900295-1900295 (2019-08-14)
A number of genes involved in zygotic genome activation (ZGA) have been identified, but the RNA-binding maternal factors that are directly related to ZGA in mice remain unclear. The present study shows that maternal deletion of Igf  2bp2 (also commonly known
Marie-Christine Birling et al.
Scientific reports, 7, 43331-43331 (2017-03-08)
Modelling Down syndrome (DS) in mouse has been crucial for the understanding of the disease and the evaluation of therapeutic targets. Nevertheless, the modelling so far has been limited to the mouse and, even in this model, generating duplication of
Jarrod McKenna et al.
PloS one, 15(12), e0244411-e0244411 (2020-12-29)
The Egyptian or Common spiny mouse (A. cahirinus) is the first rodent species to show human-like menstruation and spontaneous decidualisation. We consider from these, and its other, human-like characteristics that this species will be a more useful and appropriate small

商品

Mouse embryo media and embryo validated reagents for transgenic mouse embryo culture

用于转基因小鼠胚胎培养的小鼠胚胎培养基及经过胚胎培养验证的试剂

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