产品名称
M16 培养基, With sodium bicarbonate and lactic acid, without penicillin and streptomycin, liquid, sterile-filtered
sterility
sterile-filtered
form
liquid
technique(s)
cell culture | embryo: suitable
impurities
endotoxin, tested
components
NaHCO3: 2.101 g/L
phenol red: 0.0106 g/L
sodium pyruvate: 0.0363 g/L
glucose: 1.0 g/L (Dextro)
shipped in
ambient
storage temp.
2-8°C
Quality Level
Analysis Note
溶液中的沉淀不影响本品性能。
该产品经过测试,能够支持单细胞小鼠胚胎发育成扩大的胚泡。对胚泡发育的最低要求是 80%。
Application
M2 和 M16 培养基是植入前期胚胎体外培养的常用培养基。它是一种改良的 Krebs-Ringer 碳酸氢盐溶液,与 Whitten′s 培养基非常相似。M16 含有丙酮酸和乳酸作为能量来源,因为植入前胚胎不能有效利用葡萄糖。
Preparation Note
添加 0.06g/L 青霉素 G 钾盐和 0.05g/L 硫酸链霉素。
存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
动植物来源培养基
此项目有
Qian-Qian Sha et al.
Nature communications, 9(1), 3477-3477 (2018-08-30)
Trimethylation of histone H3 on lysine-4 (H3K4me3) is associated with gene-regulatory elements, but its transcription-independent function in cell division is unclear. CxxC-finger protein-1 (CFP1) is a major mediator of H3K4 trimethylation in mouse oocytes. Here we report that oocyte-specific knockout
Marco Ragusa et al.
RNA biology, 16(9), 1237-1248 (2019-05-29)
Circular RNAs (circRNAs) have a critical role in the control of gene expression. Their function in spermatozoa (SPZ) is unknown to date. Twenty-eight genes, involved in SPZ/testicular and epididymal physiology, were given in circBase database to find which of them
Hong-Bin Liu et al.
Advanced science (Weinheim, Baden-Wurttemberg, Germany), 6(15), 1900295-1900295 (2019-08-14)
A number of genes involved in zygotic genome activation (ZGA) have been identified, but the RNA-binding maternal factors that are directly related to ZGA in mice remain unclear. The present study shows that maternal deletion of Igf 2bp2 (also commonly known
Marie-Christine Birling et al.
Scientific reports, 7, 43331-43331 (2017-03-08)
Modelling Down syndrome (DS) in mouse has been crucial for the understanding of the disease and the evaluation of therapeutic targets. Nevertheless, the modelling so far has been limited to the mouse and, even in this model, generating duplication of
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PloS one, 15(12), e0244411-e0244411 (2020-12-29)
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