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Merck
CN

NA0410

GenElute™ HP 无内毒素质粒大提试剂盒

greener alternative

sufficient for 25 preparations

别名:

Gen Elute, GenElute 无内毒素质粒试剂盒

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NACRES:
NA.52
UNSPSC Code:
41105501

主要文件

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usage

sufficient for 25 preparations

greener alternative product characteristics

Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

technique(s)

DNA extraction: suitable

test parameters

sample volume: 150 mL bacterial culture

greener alternative category

Aligned,

storage temp.

15-25°C

Quality Level

200

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General description

Click here to view the kit protocol
GenElute HP 无内毒素质粒大量制备试剂盒可以简单、快速地从重组大肠杆菌培养物中分离不含内毒素的质粒DNA。该试剂盒采用了可快速澄清细菌裂解液的滤柱和可捕获质粒DNA的硅胶柱。配套的专利溶液可使质粒DNA结合到结合柱上，同时避免内毒素随之吸附。该技术使得用户可以维持一致的、低于 0.1 EU/μg 质粒DNA的内毒素水平。

制备所得的优质、不含内毒素DNA可直接供包括内毒素敏感细胞转染等最严格的应用使用。

Application

GenElute™ HP Endotoxin-Free Plasmid Maxiprep试剂盒已被用于分离猪肝和大肠杆菌感受态细胞中的质粒。

Biochem/physiol Actions

通过离心分离法收集过夜的重组大肠杆菌培养液，然后通过改性SDS 碱裂解法进行处理。过滤澄清裂解液，然后加入针对无内毒素质粒制备而优化的结合溶液。随后，质粒捕获到硅胶膜上，同时避免内毒素随之吸附到膜上。通过两遍洗涤步骤，去除污染物。最后，在不含内毒素的水中洗脱结合所得的DNA。回收的质粒DNA主要以超螺旋形式存在。所含的基因组DNA和RNA均低于溴化丙啶染色琼脂糖凝胶电泳的检测水平。

Features and Benefits

  • 可纯化出多达1.2 mg内毒素水平≤0.1 EU/μg高拷贝数质粒DNA
  • 从沉淀细胞到获得纯化的质粒，仅需35分钟
  • 方便的真空形式

Other Notes

更多信息，请见www.sigma-aldrich.com/genelutehp。

Legal Information

GenElute is a trademark of Sigma-Aldrich Co. LLC

试剂盒组分也可单独购买

产品编号
说明
化学品安全说明书
  • R4778Red Vac Cap 5化学品安全说明书
  • C2112Column Preparation Solution 2化学品安全说明书
  • W0263Wash Solution 1化学品安全说明书
  • N7411Neutralization Solution EF化学品安全说明书
  • W4639Wash Solution 2化学品安全说明书
  • 2107E-Toxate™ Water, endotoxin, free化学品安全说明书
  • L1912Lysis Solution化学品安全说明书
  • R1149Resuspension Solution, Add RNase A before use. After addition of RNAse, store at 2-8ºC. )化学品安全说明书
  • B9560Binding Solution化学品安全说明书
  • R6148RNase A solution化学品安全说明书
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signalword

Danger

Hazard Classifications

Acute Tox. 4 Oral - Eye Irrit. 2 - Flam. Liq. 3 - Met. Corr. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

target_organs

Central nervous system

存储类别

3 - Flammable liquids

flash_point_f

77.0 °F - closed cup

flash_point_c

25 °C - closed cup

wgk

WGK 3

法规信息

低风险生物材料
危险化学品
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历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
0000476571
0000476571
0000420536
0000420536
0000380779

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Endotoxin-free Plasmid DNA Purification

GenElute HP Plasmid Maxiprep Kit isolates endotoxin-free plasmid DNA in 35 minutes, meeting industry standards.

mRNA Synthesis

Tools for plasmid preparation, in vitro transcription, mRNA purification, and LNP formulation in mRNA-based vaccine and therapeutic development.

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用于质粒制备、体外转录、mRNA 纯化和 LNP 制剂的工具，用于基于 mRNA 的疫苗和疗法开发。

Bulletin - NA0410
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