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Merck
CN

P2545

Protein A–Agarose

saline suspension

Synonym(s):

Protein A resin

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About This Item

UNSPSC Code:
41106500
NACRES:
NA.56
MDL number:
MFCD00165563

Key Documents

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Product Name

Protein A–Agarose, saline suspension

biological source

Staphylococcus aureus

form

saline suspension

extent of labeling

~2 mg per mL

matrix activation

cyanogen bromide

matrix attachment

amino

matrix spacer

1 atom

capacity

≥15 mg/mL binding capacity (human IgG)

storage temp.

2-8°C

Quality Level

200

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Application

Protein A−Agarose has been used in the immunoprecipitation
  • of protein phosphatase and tensin homolog (PTEN) protein from S63 and NIH3T3 fibroblast cells
  • of prolyl hydroxylase domain 2 N27 cells
  • of Wnt3a and sonic hedgehog (Shh) in mouse fibroblast L-cells and human embryonic kidney (HEK) 293 cells

Protein A-agarose is used for affinity chromatography, antibody purification and characterization, and protein A, G and L resins. Protein A-agarose has been used to study the effects of protein A immunoadsorption in patients with chronic dilated cardiomyopathy as well as to study multiple sclerosis and gastric cancer.

Biochem/physiol Actions

Protein A binds to Fcγ (the constant region) and Fab (antigen binding fragment) of IgG. Protein A agarose resin is commercially used for affinity based purifications.

General description

Protein A is a 42-kDa protein from Staphylococcus aureus and has five immunoglobulin binding domains.

Physical form

Suspension in 0.5 M NaCl containing preservative

Storage Class

10 - Combustible liquids

wgk

WGK 3

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Regulatory Information

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Certificates of Analysis (COA)

Lot/Batch Number
0000294988
0000290709
0000294988
0000290709
SLCL1264

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PTEN-Dependent Stabilization of MTSS1 Inhibits Metastatic Phenotype in Pancreatic Ductal Adenocarcinoma
Zeleniak AE, et al.
Neoplasia, 20(1), 12-24 (2018)
D J McCool et al.
The Biochemical journal, 302 ( Pt 1), 111-118 (1994-08-15)
Pulse-chase labelling experiments were performed using the mucin-producing colonic carcinoma cell line LS180. Cells were pulsed with [3H]threonine or [3H]glucosamine and chased in complete media without radiolabel for various lengths of time. From cell and media extracts obtained at each
Membrane targeting of palmitoylated Wnt and Hedgehog revealed by chemical probes
Gao X, et al.
Febs Letters, 585(15), 2501-2506 (2011)
D J McCool et al.
The Biochemical journal, 312 ( Pt 1), 125-133 (1995-11-15)
We have shown previously [McCool, Forstner and Forstner (1994) Biochem. J. 302, 111-118] using pulse-chase labelling of mucin with [3H]threonine that LS180 colonic tumour cells synthesize and secrete MUC2 without the addition of secretagogues. Treatment of the LS180 cells with
D J McCool et al.
The Biochemical journal, 341 ( Pt 3), 593-600 (1999-07-27)
Molecular chaperones are presumed to associate with large secretory mucin glycoproteins during their synthesis in the endoplasmic reticulum (ER), but have not been identified to date. We decided to look for possible involvement of the chaperones calreticulin (CRT) and calnexin
View All Related Papers

Related Content

Product Information Sheet - P2545
EZview Red Protein A and ANTI-FLAG M2 Affinity Gels

EZviewTM Red Protein A and ANTI-FLAG® M2 Affinity Gels: Immunoprecipitation with Enhanced Visibility Affinity Beads - Technical Article - July 2001

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