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Merck
CN

QR0200

定量 RT-PCR ReadyMix™

One step RT-qPCR for probe-based methods, MMLV & hot-start Taq

别名:

1 步法 RT-qPCR 混合液, 实时定量 PCR 预混液

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关于此项目

NACRES:
NA.55
UNSPSC Code:
41106300

主要文件

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usage

sufficient for 250 reactions

feature

dNTPs included, hotstart

technique(s)

RT-qPCR: suitable, qPCR: suitable

Quality Level

300

color

colorless

input

purified RNA

compatibility

ABI 5700, ABI 7000, ABI 7300, ABI 7500 Fast, ABI 7500, ABI 7700, ABI 7900 HT , ABI 7900 HT Fast, ABI 7900, ABI StepOne, ABI StepOnePlus, ABI ViiA 7, Bio-Rad CFX384, Bio-Rad CFX96, Bio-Rad MJ Chromo4, Bio-Rad MJ Opticon 2, Bio-Rad MJ Opticon, Bio-Rad MiniOpticon, Bio-Rad MyiQ, Bio-Rad iCycler iQ, Bio-Rad iQ5, Cepheid SmartCycler, Eppendorf® Mastercycler ep realplex2 s, Eppendorf® Mastercycler ep realplex, Illumina Eco qPCR, Qiagen Corbett Rotor-Gene 3000, Qiagen Corbett Rotor-Gene 6000, Qiagen Corbett Rotor-Gene Q, Roche LightCycler 480, Strategene Mx3000P, Strategene Mx3005P, Strategene Mx4000

detection method

probe-based

shipped in

wet ice

storage temp.

−20°C

相关类别

General description

定量RT-PCR ReadyMix™是设计用于测量基因表达的一步式RT-PCR试剂盒，其结合了莫洛尼鼠白血病病毒逆转录酶（M-MLV RT）和JumpStart ™Taq DNA聚合酶。这种方便的2X预混液包括M-MLV RT、JumpStart ™Taq DNA聚合酶、纯度99％的脱氧核苷酸、缓冲液、玻璃钝化剂和稳定剂。JumpStart ™Taq DNA聚合酶是一种抗体灭活的热启动酶（hot-start enzyme）。一旦反应温度达到70°C，DNA聚合酶-抗体复合物随即解离，Taq DNA聚合酶活性得以恢复。这种抗体-酶复合物可实现简单便捷的设置，并相对于手动热启动技术具有更低的污染风险。该试剂盒经过设计可用于基于荧光杂交探针的检测方法。

Application

定量RT-PCR ReadyMix™已被用于通过定量逆转录聚合酶链反应（RT-qPCR）来定量测定特定表达基因的信使RNA（mRNA）水平。

Features and Benefits

  • 该主混合物（master mix）可确保不同反应之间的一致性和可再现性。
  • 降低了多个移液步骤的污染风险
  • 与手动或热启动（Hot Start）方法相比，减少了设置时间
  • JumpStart™Taq聚合酶可减少引物二聚体和非特异性产物的形成
  • 广泛的仪器兼容性
  • 包括一个单独的ROX参考染料瓶，用于反应标准化

Packaging

1个试剂盒足以用于每次20 μL的250次反应或每次50 μL的100次反应。

Legal Information

本品仅供研究使用。
Eppendorf is a registered trademark of Eppendorf SE
JumpStart is a trademark of Sigma-Aldrich Co. LLC
ReadyMix is a trademark of Sigma-Aldrich Co. LLC

仅试剂盒组分

产品编号
说明
  • Probe Based qRT-PCR ReadyMix 2 X
  • Moloney Murine Leukemia Viral Reverse Transcriptase (M-MLV RT) 5000 U

试剂盒组分也可单独购买

产品编号
说明
化学品安全说明书
  • P219210X PCR Buffer, Optimized for routine PCR with MgCl2 included 1.5 mL/vial化学品安全说明书
  • M8787Magnesium chloride solution, PCR Reagent, 25 mM MgCI2 solution for PCR 25 mM化学品安全说明书
  • P219210X PCR Buffer, Optimized for routine PCR with MgCl2 included 100 X化学品安全说明书

存储类别

12 - Non Combustible Liquids

pictograms

Exclamation markEnvironment
GHS07,GHS09

wgk

WGK 3

signalword

Danger

Hazard Classifications

Aquatic Acute 1 - Aquatic Chronic 1 - ED ENV 1 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1

法规信息

含少量动物源组分生物产品
常规特殊物品
此项目有

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
SLCH0651
SLCG8790
SLCG9928
SLCG4184
SLCG4181

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Validation of Quantitative PCR Assays
Lovatt, A., et al.
BioPharm., 22-32 (2002)
A screen of shRNAs targeting tumor suppressor genes to identify factors involved in A549 paclitaxel sensitivity
Ji D, et al.
Oncology Reports, 18(6), 1499-1505 (2007)
An Integrated High-Throughput System for mRNA Purification and Quantitation for Use in Identifying Gene Knockdown by RNA Interference
Wang, et al.
JALA: Journal of the Association for Laboratory Automation, 11, 314-318 (2006)
S A Bustin
Journal of molecular endocrinology, 29(1), 23-39 (2002-08-30)
The fluorescence-based real-time reverse transcription PCR (RT-PCR) is widely used for the quantification of steady-state mRNA levels and is a critical tool for basic research, molecular medicine and biotechnology. Assays are easy to perform, capable of high throughput, and can
T B Morrison et al.
BioTechniques, 24(6), 954-958 (1998-06-19)
Continuous fluorescence observation of amplifying DNA allows rapid and accurate quantification of initial transcript copy number. A simple and generic method for monitoring product synthesis with the double-stranded DNA dye, SYBR Green I provides initial template copy number estimation limited

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实验方案

Reverse Transcription Protocol (One-step Probe Detection)

Reverse transcription (RT) is the process of converting RNA to cDNA using a reverse transcriptase enzyme and dNTPs.

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