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Merck
CN

T0665

holo-Transferrin human

powder, BioReagent, suitable for cell culture, ≥97%

Synonym(s):

Siderophilin, iron-saturated

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About This Item

CAS Number:
UNSPSC Code:
12352202
EC Number:
234-318-8
NACRES:
NA.75
MDL number:
eCl@ss:
34058011
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biological source

human plasma

Quality Level

description

Iron-saturated

product line

BioReagent

assay

≥97%

form

powder

technique(s)

cell culture | mammalian: suitable

impurities

HIV, HBsAg and HCV, none detected (tested by FDA approved testing methods), endotoxin, tested

solubility

H2O: 50 mg/mL

UniProt accession no.

shipped in

ambient

storage temp.

2-8°C

Gene Information

human ... TF(7018)

Application

holo-Transferrin human has been supplemented in Roswell Park Memorial Institute (RPMI) for proerythroblasts proliferation assay and also in culture media for inductively coupled plasma emission spectroscopy.
Transferrin is the primary iron binding and cell delivery molecule present in serum. Serum-free cell culture systems require a delivery format for iron. Transferrin is the preferential delivery form of iron because cells process transferrin bound iron in a physiologically appropriate way through transferrin receptors on the cell surface. Human holo-transferrin is a high affinity transferrin that can be used with a wide range of cells types. Human holo-transferrin is loaded with iron prior to use and can be added directly to iron poor or iron-free cell culture media.

Biochem/physiol Actions

Provides iron to cells in a physiologically stable and safe form.

Preparation Note

Heat treated at 60 °C (minimum) for a minimum of 10 hours.

Analysis Note

Iron content by ICP reported on a lot-specific basis.
Purity by agarose gel electrophoresis.

Disclaimer

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.


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Storage Class

11 - Combustible Solids

wgk

WGK 3

Regulatory Information

高风险级别生物产品--人源产品

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Jill M Brown et al.
Nature communications, 9(1), 3849-3849 (2018-09-23)
Self-interacting chromatin domains encompass genes and their cis-regulatory elements; however, the three-dimensional form a domain takes, whether this relies on enhancer-promoter interactions, and the processes necessary to mediate the formation and maintenance of such domains, remain unclear. To examine these
Co-operative signalling mechanisms required for erythroid precursor expansion in response to erythropoietin and stem cell factor
Arcasoy O and Jiang X
British Journal of Haematology, 130(1), 121-129 (2005)
Senthil Raja Jayapal et al.
Cell cycle (Georgetown, Tex.), 15(22), 3070-3081 (2016-09-23)
Cyclin A2 is an essential gene for development and in haematopoietic stem cells and therefore its functions in definitive erythropoiesis have not been investigated. We have ablated cyclin A2 in committed erythroid progenitors in vivo using erythropoietin receptor promoter-driven Cre



Global Trade Item Number

SKUGTIN
T0665-100MG04061837335662
T0665-1G04061837335679
T0665-500MG04061837335709
T0665-50MG04061837335723