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Merck
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TRUE2

TrueGel3D 水凝胶试剂盒

FAST-DEXTRAN, allow cell recovery, PEG non cell-degradable crosslinker

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关于此项目

NACRES:
NA.71
UNSPSC Code:
12352207
Storage temp.:
−70°C
Shipped in:
dry ice
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shipped in

dry ice

storage temp.

−70°C

General description

The gel is formed by stable thioether bonds between maleimide groups of dextran (FAST-DEXTRAN) and thiol groups on PEG non cell-degradable crosslinker. Maleimide-functionalized dextran is specifically used to decrease the time for gel formation. The dextran polymer does not have cell attachment sites, but it can be customized to conjugate RGD peptide using TrueGel3D RGD (TRUERGD-1UM) prior to the crosslinking step.

TrueGel3D enzymatic cell recovery solution (TRUEENZ-500UL) is used to dissolve dextran polymers for easy recovery of chemically fixed or live cells for post-culture analyses including RT-PCR, western blots and other molecular biological studies.

Application

TRUE2-1KT is used to grow cells that don′t attach to hydrogel for survival but rather use their secreted extracellular matrix. It is used to immobilize cells or specimens for imaging and for bioprinting.

Features and Benefits

  • Fast gelling (takes few seconds to minutes after addition of crosslinker)
  • Allow cell recovery and post-culture analyses
  • Flexibility in modulating gel stiffness
  • Cells do not attach to hydrogel (if not customized with TrueGel3D RGD peptide)
  • Does not allow cell migration

Other Notes

TRUE2-1KT contains lyophilized FAST-DEXTRAN (polymer) and PEG non cell-degradable crosslinker with reconstitution buffer, TrueGel3D thioglycerol, and water.

Disclaimer

Avoid prolong exposure of PEG non cell-degradable crosslinker to air.


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pictograms

Corrosion

signalword

Warning

hcodes

Hazard Classifications

Met. Corr. 1

存储类别

10 - Combustible liquids



历史批次信息供参考:

分析证书(COA)

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实验方案

An advanced protocol is available for TrueGel 3D kits for 3D cell culture for peptides concentration or stiffness optimisation

TrueGel3D hydrogel enables cell encapsulation for 3D culture, mimicking native tissue environment with chemically defined properties.

商品

Hydrogela are the most widely used systems for 3D cell culture. Learn more about this technology (what are hydrogels? How to chose?)

TrueGel3D™ is a synthetic hydrogels platform for 3D cell culture that aim to mimic the natural extracellular environment for more physiological culture conditions

Troubleshoot TrueGel3D™ hydrogel formation and cell viability issues with RGD-degradable polymer and CD cell-degradable crosslinker.

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全球贸易项目编号

货号GTIN
TRUE2-1KT04061835539253