Intensify your seed train
Solutions to increase cell density and improve process efficiency in your upstream bioprocess.
Seed Train Platform
Future-proof your manufacturing with tailored solutions for seed train intensification
Seed train intensification allows for significantly higher cell densities compared to traditional batch processes. This is achieved through methods such as using perfusion processes, or integrating cryopreservation techniques to streamline the inoculation of production bioreactors.
- Cellicon® Cell Retention Solution separates and retains cells in a culture while allowing the surrounding media and waste products to pass through, maintaining high cell densities, improving cell viability, and facilitating continuous production.
- Mobius® High Cell Density Cryopreservation Assemblies enable seed train inoculation of cells at higher densities, ( > 50 x 106 cells/mL) and larger volumes and allows for direct inoculation of bioreactors.
Try it yourself! By submitting this webform, you will receive a free sample of the HCDC Bag Assembly (-80 °C) 100 mL or 250 mL, or HCDC Bag Assembly (-196 °C) 100 mL or 250 mL. Take the opportunity to also request a demo of the Cellicon® Cell Retention Solution.
Cellicon® Cell Retention Solution: High Throughput and Reproducible Performance
The Cellicon® Cell Retention Solution includes a lab-scale flat sheet filter assembly and controller for perfused seed trains, featuring an intuitive piping and instrumentation diagram (P&ID) for process visualization. The process-scale version, with Mobius® Cell Retention System and Bio4C ACE™ software, offers scalable sizes (50–2000 L) ensuring easy integration with bioreactors. They offer:
- High throughput and low fouling
- Low crossflow and cell shear
- Predictable linear scalability from lab to manufacturing scale
- Reliable and reproducible performance
- Comprehensive monitoring and precise control
They are easy to use, install and are ready to process in minutes.
Mobius® High Cell Density Cryopreservation Assemblies: Lower Risk of Contamination and Improved Reproducibility
These assemblies are specifically designed to facilitate the freeze and thaw of cryopreserved cells in either ultra-low temperature (-80 °C format) or liquid nitrogen (-196 °C format) freezers. Both formats are available in two assembly bag sizes of 100 mL and 250 mL in a 5-bag manifold. This multi-bag design:
- Significantly reduces environmental contamination risk compared to vials or single bag operations
- Improves process reproducibility by providing uniform inoculation feed across multiple campaigns
Discover more on the Seed Train Platform and how to intensify your upstream process.
Related Resources
- Operational and Financial Advantages of Cellicon® Cell Retention Solution in an N-1 Perfusion Process
The following study compares an N-1 perfusion process at the 2,000 L scale using our Cellicon® Cell Retention Solution versus a commercially available cell retention device.
- Reproducible Scaling of a Cell Retention Filter Using the Cellicon® Cell Retention Solution
This page describes the design attributes of the Cellicon® Filter Assembly and how they simplify process scale-up.
- Seed Train Intensification Using High Cell Density Cryopreservation
This article explains the benefits of high cell density cryopreservation (HCDC) and how to implement it.
- Impact of DMSO And Freezing Technique in Developing a High Cell Density Cryopreservation Process for Upstream Bioprocessing
Here, we evaluated how dimethyl sulfoxide and freezing techniques impact viable cell density and titer production in an HCDC intensified seed train.
- Cell Retention Technology and Specialized Cell Culture Media for Intensified Upstream Processing
This study demonstrates the use of a Cellicon® filter and controller in an N-1 perfusion process with Cellvento® 4CHO-X expansion medium as compared to a control seed train using shake flasks.
REQUEST A SAMPLE/DEMO
By submitting this webform, you will receive a free sample of an HCDC Bag Assembly (1 assembly), or a demo of the Cellicon® Cell Retention Solution.
See Official Rules
To continue reading please sign in or create an account.
Don't Have An Account?