Home WebinarsIntegration of Automated Sampling with LC-MS as a PAT Tool for USP Glycsoylation Monitoring and Control

Integration of Automated Sampling with LC-MS as a PAT Tool for USP Glycsoylation Monitoring and Control

WEBINAR

This webinar explores how automation strategies and control schemes facilitate rapid analytical results, crucial for managing glycosylation variability that affects product quality. The MAST^® Autosampling Solution and Agilent 2D-LC/TOF MS was used to enable glycosylation monitoring during perfusion cell culture. A digital lab infrastructure and cloud-based orchestration layer managed communication and control between the units, allowing easy modification of parameters like target galactosylation and sampling frequency. Over a 35-day culture process, targeted galactosylation setpoints were maintained within a ± 2% deadband, ensuring efficient data collection and timely insights.

In this webinar, you will learn:

Current limitations in conventional sampling and analytical methods
The role of automated sampling in enhancing process control
How digital technologies and PAT are integrated to drive bioprocess innovation
About case studies for on-line glycosylation monitoring

Speaker

Srijana Chapagain

Merck

PAT & Digital Applications

Srijana Chapagain is an applications engineer within the single-use and integrated systems PAT (Process Analytical Technology) & digital applications team. She joined Merck in 2020 and was previously a part of the SU&IS upstream applications team. In her role, Srijana has been involved in various product development initiatives and technology assessments within the upstream bioprocessing and (PAT)-enabling sector. Her current focus encompasses PAT integration and the digital transformation of laboratory operations. Srijana holds a B.Sc. in chemical engineering from Villanova University and a M.Sc. in engineering management from Tufts University.