Home WebinarsEnabling On-Line CQA Analysis via Autosampling: Real-Time Glycosylation Control in Perfusion

Enabling On-Line CQA Analysis via Autosampling: Real-Time Glycosylation Control in Perfusion

WEBINAR

In this webinar, you will learn how:

Autosampling may be integrated with 2D-LC/TOF MS for real-time titer and glycosylation analysis
PID controllers may be configured to automatically adjust feed rates based on real-time analytical results
Robust control performance can be achieved across 30+ day perfusion runs under intentional process perturbations
To leverage a plug-and-produce PAT infrastructure to streamline data workflows for lab teams and accelerate digitalization for biopharma development

Glycosylation variability in mAb bioprocessing significantly impacts product quality, yet conventional sampling takes days to weeks for results. An integrated on-line system combining MAST^® autosampling with Agilent 2D-LC/TOF MS for real-time titer and glycoform analysis was developed. A cloud-based orchestration layer and PID controller automatically adjust feed media (Cellvento^® ModiFeed Prime Gal+/-) based on LC/MS results, maintaining glycosylation targets within ±2% deadband.

Across multiple perfusion runs, the system demonstrated robust control despite intentional perturbations, with automated samples being analyzed within one hour. Titer and glycosylation results correlated excellently with off-line analysis, enabling real-time CQA control without manual intervention and sampling workflows.

Speaker List

Matthew Wong

Merck

Applications Engineer

Matthew is an Engineer III in the PAT and Digital Applications team in Single-Use and Integrated Process Solutions R&D at our company. His work focuses on leveraging LCMS for online process analytics, such as monitoring mAb titer and glycosylation. He is also involved in digital network integration to enable advanced control and data-driven insights. Matthew earned his B.S. in Chemical and Biomolecular Engineering from the University of Delaware and his Ph.D. in Chemical and Biological Engineering from Rensselaer Polytechnic Institute.

Isabel Dunning

Merck

Applications Engineer

Isabel joined the company in July 2021 and is currently an Upstream R&D scientist working within the Single Use and Integrated Systems Applications group in Bedford, MA. The objective of her role is to implement PAT & Digital technologies into the upstream process to optimize cell culture performance, improve day-to-day operations, automize processes, and increase robust data collection. Isabel studied Biomedical Sciences and Immunology at Quinnipiac University and received her MS in Biotechnology and Business Management at the University of Wisconsin, Green Bay.

Mike Knierman

Agilent

Biopharma Workflow Manager

Mike Knierman is an expert in the analysis of proteins. Prior to his current role at Agilent, he worked at Eli Lilly for 26 years in research and development and at NIH for 5 years in peptide synthesis. Mike’s work has led to 55 publications and 4 patents in many diverse areas such as COVID-19, Alzheimer’s disease, Parkinson’s disease, diabetes, immunology, and biomarker discovery and validation. He has designed several mass spectrometry interfaces for nanoLC and capillary electrophoresis and has also developed several software pipelines for the analysis of mass spectrometry data. Today as the Biopharma Workflow Manager at Agilent, he oversees the biopharma workflows and creates new directions for Agilent in this space. Mike is passionate about scientific equipment and helping people live better lives.