Molecular Biology & Genomics Learning Hub

High-quality custom DNA oligos made to meet your specifications (scale, purification, length, dry/solution, modifications, tubes/plates).

After a traditional PCR has been completed, the PCR/qPCR data analysis is conducted by resolution through an agarose gel or, more recently, through a capillary.

Quantitative PCR detection builds on basic PCR techniques and allows researchers to estimate the quantity of starting material in a sample with a much wider dynamic range.

Polymerase chain reaction is one of the most widely used techniques in molecular biology with 3 main steps: denature, anneal, and extend.

The T7 promoter is commonly used to regulate gene expression of recombinant proteins, which can be subsequently used for a variety of downstream research applications.

Overview of common techniques and downstream applications for extraction and purification of genomic DNA, plasmid DNA, and total RNA from cells, blood, viruses, and more.

Epigenetics tools and techniques for researching the complexities of epigenetic mechanisms including DNA methylation, histone modification, and RNA regulation.

Quantitative PCR (qPCR) or quantitative Real-Time PCR applications, utilizing fluorescent report molecules, suitable for your microRNA, genotyping, and gene expression analyses.

Learn the basics of DNA and RNA gel electrophoresis, and how these techniques can be used to separate and purify DNA and RNA molecules based on size and charge.

CRISPR, miRNA, siRNA, and shRNA technologies for gene expression and gene silencing applications.

Learn about Sanger Sequencing steps or the chain termination method and how DNA sequencing works and how to read Sanger Sequencing results accurately for your research.

Amino Acid Codon Wheel for fast RNA translation. Find which amino acid is translated from your RNA sequence quickly and easily.

Learn standard PCR protocol steps and review reagent lists or cycling parameters. This method for routine PCR amplification of DNA uses standard Taq DNA polymerase.

PCR troubleshooting guide for reliable data in molecular assays.

Our SYBR Green qPCR Protocol is a method designed to detect accurate quantification of gene expression and RT-PCR reactions.