Liver Powder, dehydrated

To prepare a liver infusion medium, add 50 g of liver powder to 1 litre of distilled water and allow to infuse (with frequent agitation) for 1 hour at 50°C. Boil the mixture for a few minutes to coagulate protein, strain through 60 mesh stainless steel gauze, add 10 g of peptone and 5 g of sodium chloride. Adjust the reaction to pH 7.2, boil, strain through gauze as above, and sterilize by autoclaving at 121°C for 15 minutes.