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P8651

Protein A–Peroxidase from Staphylococcus aureus/horseradish

lyophilized powder

Synonym(s):

Peroxidase–Protein A

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.46
MDL number:
MFCD00132102

Key Documents

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Product Name

Protein A–Peroxidase from Staphylococcus aureus/horseradish, lyophilized powder

conjugate

peroxidase conjugate

form

lyophilized powder

technique(s)

direct ELISA: 1:80,000 using human IgG

storage temp.

−20°C

Quality Level

100
200

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Related Categories

Application

Protein A–Peroxidase from Staphylococcus aureus/horseradish has been used in enzyme-linked immunosorbent assay (ELISA).

Biochem/physiol Actions

Binds IgG only from most mammals, except rat, goat, sheep.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Protein A is a cell wall constituent of Staphylococcus aureus, with a molecular weight of 56kDa. It possesses five binding domains which interact with heavy chains of immunoglobulins at the Fc or Fab fragment. In response to protein A, the complement system, interferon production, hypersensitivity reactions and cell-mediated cytotoxicity get activated. Horseradish peroxidase is a heme-containing enzyme. In the presence of hydrogen peroxide, the enzyme catalyzes the oxidation of luminol.

Packaging

Package size based on protein content.

Physical form

Lyophilized powder containing sodium citrate buffer salt. Protein determined by A205.

Preparation Note

Protein A, extracellular, coupled to Peroxidase, Type VI, by a modification of the procedure of O′Sullivan, M.J., et al., FEBS Lett., 95, 311 (1978), which favors low molecular weight conjugates.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Regulatory Information

常规特殊物品
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Certificates of Analysis (COA)

Lot/Batch Number
0000329693
0000329693
SLCP2555
SLCH3263
SLCG0737

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I prepare a stock solution of Product P8651, Protein A Peroxidase?

    A stock solution of this product can be prepared in PBS (phosphate buffered saline) or other neutral buffer at 1 mg/mL.

  4. What is the solution stability of Product P8651, Protein A Peroxidase?

    The solution should be stable for about one week when stored at 2-8°C. For long-term storage, the stock solution should be frozen in aliquots at -20 °C.

  5. How do I use Product P8651, Protein A Peroxidase, as a my secondary in Western blot analysis?

    For Western blotting, with chemiluminescent substrate, dilute 1:1000 to 1:10,000.

  6. I bought Product P8651, Protein A Peroxidase, to hide heavy and light chain in a Western blot. How does it do that?

    P8651 will only recognize the native antibody incubated on a blot, not what's run in the gel. This should circumvent heavy and light chain detection.

  7. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  8. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  9. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Florina Vlad et al.
The Plant cell, 21(10), 3170-3184 (2009-10-27)
The plant hormone abscisic acid (ABA) orchestrates plant adaptive responses to a variety of stresses, including drought. This signaling pathway is regulated by reversible protein phosphorylation, and genetic evidence demonstrated that several related protein phosphatases 2C (PP2Cs) are negative regulators
Identification of a Complex of the Three Forms of the Rat Liver
Asialoglycoprotein Receptor
James T. Sawyer
The Journal of Biological Chemistry, 263 (1998)
Sudha Thoennes et al.
Virology, 370(2), 403-414 (2007-10-16)
Influenza virus entry occurs in endosomes, where acidification triggers irreversible conformational changes of the hemagglutinin glycoprotein (HA) that are required for membrane fusion. The acid-induced HA structural rearrangements have been well documented, and several models have been proposed to relate
Horseradish peroxidase: a modern view of a classic enzyme
Nigel C
Phytochemistry, 65(3) (2004)
Maria Fort et al.
Veterinary microbiology, 125(3-4), 244-255 (2007-07-06)
The notion that postweaning multisystemic wasting syndrome (PMWS)-affected pigs develop an impaired humoral response against porcine circovirus type 2 (PCV2) has been reported in several studies. However, little information is available regarding the presence of neutralizing antibodies (NA) in PCV2-infected
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Protocols

Enzymatic Assay of Peroxidase (EC 1.11.1.7)

This procedure is for the determination of Peroxidase enzymatic activity using Pyrogallol as the substrate.

Enzymatic Assay of Peroxidase (EC 1.11.1.7) 2,2'-Azino-bis(3-Ethylbenzthiazoline-6-Sulfonic Acid) as a Substrate

To standardize a procedure for the assay of Peroxidase using 2,2'-Azino-bis(3-Ethylbenzthiazoline-6-Sulfonic Acid) as a substrate.

Spectrophotometric Determination of Reinheitszahl (RZ) for Peroxidase (EC 1.11.1.7)

Reinheitszahl (RZ) is the ratio of absorbance due to hemin (A403, Soret region) to absorbance due to protein (A275).

过氧化物酶的酶学测定（EC 1.11.1.7）

该实验程序用于使用连苯三酚作为底物测定过氧化物酶的酶活性。

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